猪瘟病毒内蒙流行株E2融合基因的构建

目的：获取猪瘟病毒内蒙流行株的E2基因并构建融合基因。方法：采用逆转录聚合酶链反应(RT-PCR)技术从感染猪瘟病毒内蒙流行株猪脾脏中获得并扩增E2基因特异性片段，将扩增的E2基因测序后与Chaperone10相连接构成融合基因并连接到表达载体上。结果：所获得的猪瘟病毒内蒙流行株E2基因和其他地区猪瘟病毒流行株(HCLV株)E2基因有4个碱基的差异；融合基因Chaperone10-E2构建成功。结论：不同地区猪瘟病毒E2基因差异不大。

Construction of E2 fusion gene from Neimeng strain of swine fever virus

Objective To obtain the E2 genes from Neimeng strain of classical swine fever virus and construct fusion genes. Methods E2 DNA fragment was acquired from spleen of infected pig and amplified through RT PCR technique. After being sequenced, the E2 gene was ligated to Chaperone 10 to form the fusion gene Chap 10 E2, the fusion gene was ligated to expressing vector. Results There were differences in four bases between E2 gene from Neimeng strain of swine fever virus and E2 gene from HCLV strain. The fusion gene Chap 10 E2 was successfully constructed. Conclusion The difference of E2 gene of swine fever virus from different strains is not significant.