辛伐他汀对C反应蛋白诱导的单核细胞白细胞介素-6合成的影响

目的　探讨辛伐他汀对C反应蛋白 (C reactiveprotein ,CRP)诱导的人外周血单核细胞白细胞介素 6 (interleu kin 6 ,IL 6 )合成的影响 ,观察辛伐他汀的抗炎作用。方法　密度梯度离心法分离人外周血单核细胞 ,应用酶联免疫吸附测定法观察CRP刺激单核细胞产生IL 6的时间 剂量效应 ,其峰值与辛伐他汀抑制剂组比较。结果　CRP刺激IL 6合成开始的时间是 4h ,呈时间依赖性和剂量依赖性 ,在 2 4h达高峰。辛伐他汀 (10 - 8～ 10 - 6 mol L)呈剂量依赖性抑制单核细胞IL 6合成。结论　辛伐他汀能抑制CRP诱导的单核细胞IL 6合成 ;辛伐他汀对炎症反应的抑制作用提示该药物可用于预防和治疗冠心病。

Effects of simvastatin on interleukin-6 synthesis in monocytes stimulated by C-reactive protein

Objective To evaluate the effect of simvastatin on interleukin-6 (IL-6) production in human monocytes stimulated by C-reactive protein(CRP) and to assess the influence of simvastation on monocyte inflammatory reaction.Methods Monocytes were isolated by density gradient method from blood of healthy volunteers.IL-6 production(by ELISA) stimulated by 20 μg/ml CRP for 2, 4, 8, 16 and 24 h or by 1 μg/ml, 5 μg/ml, 10 μg/ml, 20 μg/ml CRP for 24 h was measured The peaks were compared with those in the group inhibited by simvastatin(10 -8 to 10 -6 mol/L).Results IL-6 was produced after stimulation with CRP for 4 h. The effects of CRP were dose-dependent and time-dependent, reaching peak at 24 h. Simvastatin (10 -8 to 10 -6 mol/L) inhibited the production of IL-6 in a dose-dependent manner.Conclusion Simvastatin can inhibit the production of IL-6,suggesting that it can be used in clinical prevention and treatment of coronary heart disease.