壮骨颗粒对骨关节炎兔去分化软骨细胞影响的实验研究

目的:探讨壮骨颗粒对膝骨性关节炎兔软骨细胞增殖、凋亡的影响。方法:60只新西兰大白兔用Hulth法造骨关节炎动物模型.随机分成空白对照组、实验组:实验组用壮骨颗粒灌胃,空白对照组用生理盐水.治疗第4、8、12、24周后每绢各取6只动物.用亚硝酸盐还原酶法测定关节滑液NO水平.关节软片组织HE染色,光镜观察形态学变化、电镜观察软骨细胞内结构变化及原位末端标记(TLNEL)法观察软骨细胞凋亡病理改变,进行组织病理学评估。结果:空白对照组NO活性在不同时期均高于实验组(8周.P<0.05;12、24周,P<0.01),两组问比较有显著性差异P<0.05,0.01):实验组的关节软骨病理改变总积分、软骨细胞病理改变积分和软片表层病理改变积分均明显低于空白对照组(P<0.05);实验组的软骨细胞凋亡指数与对照组的软骨细胞凋亡有显著性差异(P<0.05)。与关节液中NO含量显著相关。结论:壮骨颗粒对兔膝关节实验性骨关节炎软骨退变具有保护作用。通过NO诱导途径减少软骨细胞的凋亡。可能是其作用机制之一。

Experimental Study of Effect Zhuanggu Granula to Rabbit Osteoarthritis Chondrocyte

Objective:To investigate Zhuanggu granula affecting chondrocyte multiplication apoptosis.Methods:60 rabbits were made model by Hulth,and divided into two groups,vacant group and treating group at 4 weeks;8 weeks;12 weeks;24 weeks,Both groups were executed and draw the materials from rabbits.Chemical examining,observating by light microscope and electron microscope.In situ end-labeling observing chondrocyte apoptosis was made(8 weeks P<0.05,24 weeks P<0.01).Conclusions:Zhuanggu granula could protecting cataplasia chondrocyte by NOinducing decrease chondrocyte apoptosis.It maybe one mechanism of action.