Communication between human mast cells and CD4+ T cells through antigen‐dependent interactions |
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Authors: | Jolien Suurmond Jurgen van Heemst Jacqueline van Heiningen Annemarie L Dorjée Marco W Schilham Feddo B van der Beek Tom WJ Huizinga Annemie JM Schuerwegh René EM Toes |
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Institution: | 1. Department of Rheumatology, Leiden University Medical Center, , Leiden, The Netherlands;2. Department of Pediatrics, Leiden University Medical Center, , Leiden, The Netherlands;3. Department of Otorhinolaryngology, Leiden University Medical Center, , Leiden, The Netherlands;4. Department of Immunology/Allergology/Rheumatology, University of Antwerp, , Antwerp, Belgium |
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Abstract: | Mast cells (MCs) are immune cells residing in tissues where pathogens are first encountered. It has been indicated that MCs might also be involved in setting the outcome of T‐cell responses. However, little is known about the capacity of human MCs to express MHC class II and/or to capture and present antigens to CD4+ T cells. To study the T‐cell stimulatory potential of human MCs, CD34+ stem cell derived MCs were generated. These cells expressed HLA‐DR when stimulated with IFN‐γ, and, importantly, presented peptide and protein for activation of antigen‐specific CD4+ T cells. The interplay between MC and T cell led to increased HLA‐DR expression on MCs. MCs were present in close proximity to T cells in tonsil and expressed HLA‐DR and CD80, indicating their ability to present antigens to CD4+ T cells in T‐cell areas of human LNs. Our data show that MCs can present native antigens to human CD4+ T cells and that HLA‐DR expressing MCs are present in tonsil tissue, indicating that human MCs can directly activate T cells and provide a rationale to study the potential of MCs to prime and/or skew human T‐cell responses. |
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Keywords: | Antigen presentation CD4+ T lymphocytes Mast cell |
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