慢性胃病患者口腔与胃内幽门螺杆菌研究

目的：研究慢性胃病患者口腔中是否存在幽门螺杆菌（Hp），并分析胃病患者牙周状况与幽门螺杆菌的关系。方法：依据特异的尿素酶C基因和cagA基因设计引物，建立聚合酶链反应（PCR）方法。检测65例慢性胃病患者不同牙位龈上和龈下菌斑中的Hp。结果：65例慢性胃病患者胃黏膜标本中，PCR检测阳性标本58例（89．3％），龈下菌斑Hp阳性率（47．7％），高于龈上菌斑中的Hp阳性率（26．2％）。牙周袋深度（PD）≥4mm部位菌斑中Hp检出率显著高于PD〈4mm的部位（P〈0．05）。结论：口腔Hp是慢性胃病患者Hp感染的重要来源。

Study of Helicobacter pylori in oral cavity and stomach from chronic gastritis patients

Objective: To investigate the prevalence of Helicobacter pylori(Hp)in the oral cavity of gastritis patients, and analyze the relationship of the patients' chronic periodontal disease and Hp.Method: A polymerase chain reaction (PER)assay with oligonucleotide primers homoologous to a portion of the urease C and cytotoxin-associated gene A(cag A)of Hp was used to test for the presense of Hp in dental plaque.65 gastric disease patients were recruited to detect Hp in supra-and subgingival plaque.Result: 65 chronic gastritis patients,58 patients(89.3%)had at least one lip positive dental plaque sample.The Hp positive rate was significantly higher in supragingival plaque(47.7%)than in suhgingival plaque(26.2%).The prevalence of Hp was positively correlated with probing depth.Conclusion: Oral Hp infection may be an important source of gastric Hp infection.