Genomic organization of a cellulase gene family in Phanerochaete chrysosporium |
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Authors: | Sarah F. Covert Jennifer Bolduc Dan Cullen |
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Affiliation: | (1) Department of Bacteriology, University of Wisconsin-Madison, 53706 Madison, WI, USA;(2) Institute for Microbial and Biochemical Technology, Forest Products Laboratory, Forest Service, U.S. Department of Agriculture, 53705-2398 Madison, WI, USA;(3) Present address: Department of Plant Pathology, University of Arizona, 85721 Tucson, AZ, USA |
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Abstract: | Summary Southern blot and nucleotide sequence analysis of Phanerochaete chrysosporium BKM-F-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the Trichoderma reesei cellobiohydrolase I gene (cbh1). Using pulsed-field gel electrophoresis to separate P. chrysosporium chromosomes, the six cellulase genes were found to hybridize to at least three different chromosomes, one of which is dimorphic. The organization of these genes was similar in another P. chrysosporium strain, ME 446. It is clear that, unlike T. reesei, the most well-studied cellulolytic fungus, P. chrysosporium contains a complex, cbh1-like gene family. |
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Keywords: | Gene family Cellulase genes Cellobiohydrolases Clamped homogeneous electric field |
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