微囊化异种嗅球组织细胞悬液移植脊髓损伤大鼠核因子κB表达的变化

背景：研究发现,微胶囊包裹异种嗅球组织细胞可以减少免疫排斥反应,促进损伤脊髓的功能修复,但其作用途径尚不清楚。 目的：观察微囊化异种嗅球组织细胞移植于脊髓损伤大鼠后核因子κB的表达及活性变化。 方法：家兔用于制备异种嗅球组织细胞悬液。SD大鼠分为4组,即假手术组、微囊组、细胞组及单纯损伤组,后3组在建立大鼠脊髓半横断损伤模型后分别于损伤处植入明胶海绵吸附的10 μL微囊化异种嗅球组织细胞、10 μL异种嗅球组织细胞以及10 μL生理盐水。采用苏木精-伊红染色观察脊髓组织的病理学改变,免疫组织化学染色观察核因子κB的表达变化。 结果与结论：脊髓损伤后大鼠神经元细胞浆及细胞核内核因子κB表达增加,24 h达高峰水平,3 d后开始逐渐降低,7 d基本降至正常。微囊组脊髓核因子κB阳性细胞数少于单纯损伤组及细胞组(P < 0.05)。提示微囊化异种嗅球组织细胞移植对脊髓损伤后核因子κB的活性表达起抑制作用,有益于减轻核因子κB介导的炎性反应。

Effects of the xenotransplantation of microencapsulated olfactory bulb cell suspension on the expression of nuclear factor-kappa B in rats after spinal cord injury

BACKGROUND:Studies show that the microencapsulated heterogeneous olfactory bulb cells can reduce the immunological rejection and improve the functional recovery of spinal cord injury. However, the mechanism is unclear. OBJECTIVE:To investigate the effects of the xenotransplantation of microencapsulated olfactory bulb cells into rats on the expression and activity of nuclear factor-kappa B (NF-κB) after spinal cord injury. METHODS:Rabbits were used to prepare the heterogeneous olfactory bulb cell suspension. Sprague Dawley rats were randomly divided into four groups: the sham operation group, the microcapsule group, the cell group and the simple injury group. The rats in the later three groups were prepared for spinal cord hemisection model and transplanted with gelatin sponge sticking 10 μL microencapsulated olfactory bulb cell suspension, 10 μL microencapsulated olfactory bulb cell suspension, and 10 μL physiological saline respectively. The pathological changes of spinal tissues were observed by haematoxylin-eosin staining, and the expressions of NF-κB were observed by immunohistochemical staining.  RESULTS AND CONCLUSION:The expressions of NF-κB in the cytoplasm and cytoblast of neurons were increased in rats after spinal cord injury. The expression level reached a peak at 24 hours, gradually decreased after 3 days, and returned to the normal level after 7 days. The NF-κB+ cells in the microcapsule group were obviously fewer than that in the cell and simple injury groups (P < 0.05). The xenotransplantation of microencapsulated olfactory bulb cells can inhibit the expression and activity of NF-κB to mitigate the NF-κB modified inflammatory reaction after spinal cord injury.