S100a7蛋白在体外培养人视网膜色素上皮细胞中的表达及功能

背景：S100a7蛋白在细胞增殖、血管形成等病理生理过程中发挥重要作用,但在视网膜色素上皮细胞中的作用少有报道。目的：探讨S100a7蛋白在体外培养人视网膜色素上皮细胞中的表达及作用。方法：体外培养ARPE-19细胞株,通过免疫荧光及Western blot技术,检测S100a7在ARPE-19细胞中的表达;不同稀释度(1∶1 000,1∶10 000,1∶100 000)S100a7蛋白抗体与ARPE-19细胞共培养,通过MTT技术检测S100a7蛋白在人视网膜色素上皮细胞增殖过程中的作用。结果与结论：免疫荧光及Western blot技术证实该蛋白在ARPE-19细胞中表达;MTT实验结果显示,人视网膜色素上皮细胞加入S100a7抗体72 h后,各浓度处理组的吸光度值较对照组显著均降低(P < 0.05)。结果证实S100a7蛋白在人视网膜色素上皮细胞中的表达,并明显促进人视网膜色素上皮细胞增殖。

Expression identification and preliminary function analysis of S100a7 in retinal pigment epithelial cells cultured in vitro

BACKGROUND: S100a7 is a new protein that participates in cell proliferation, vascularization and other pathophysiological processes.OBJECTIVE: To discuss the expression of S100a7 in retinal pigment epithelial cell (RPE) and to investigate the effects of S100a7 on RPE proliferation.METHODS: Immunofluorescence and western blot techniques were used to detect the expression of S100a7 in ARPE-19, and MTT was performed to investigate the effects of S100a7 on RPE proliferation.RESULTS AND CONCLUSION:S100a7 was proven to be expressed in ARPE-19 by immunofluorescence and western blot techniques and it could significantly promote ARPE-19 cell proliferation. This study first confirmed the expression of S100a7 in ARPE-19 cells, which may play a specific role in the pathogenesis of proliferative vitreoretinopathy.