心肌条件培养液与5-氮胞苷诱导骨髓基质干细胞向心肌样细胞的分化

背景：骨髓基质干细胞可以明显改善心肌缺血时的心脏功能，但其直接分化为心肌细胞并参与心功能恢复的机制尚不明确。目的：探讨心肌条件培养液与5-氮胞苷诱导骨髓基质干细胞分化为心肌样细胞的作用。方法：全骨髓贴壁法分离培养骨髓基质干细胞，将第6代骨髓基质干细胞随机分为4组：5-氮胞苷+心肌条件培养液联合诱导组；5-氮胞苷组；心肌条件培养液组；基础培养基组(空白组)。用心肌条件培养液和5-氮胞苷诱导3周后用免疫组化法测定各组心肌肌钙蛋白表达，并采用单因素方差分析检验进行统计学分析。结果与结论：在体外心肌条件培养液可以诱导骨髓基质干细胞向心肌细胞分化，提示其中细胞因子可能起关键作用，但心肌条件培养液的这种作用要弱于化学诱导剂5-氮胞苷的诱导作用，且联合诱导作用更强。

Induction of marrow stromal cells into myocardical cells by conditioned cultrure medium of cardiac muscle and 5-azacytidine

BACKGROUND:Bone marrow stromal cells (MSCs) can significantly improve cardiac function after ischemia-induced myocardium injury, but the mechanism of differentiation of MSCs into myocardical cells and its contribution to the regeneration of cardiac function remains uncertain.OBJECTIVE:To explore the effects of conditioned cultrure medium of cardiac muscle and 5-azacytidine on inducing MSCs into myocardial cells. METHODS:MSCs were sorted from whole bone marrow by adherent culture. MSCs of the 6th generation were divided into 4 groups: combined group (5-azacytidine + conditioned cultrure medium of cardiac muscle; 5-azacytidine group; conditioned cultrure medium group; blank group (basal culture medium). At 3 weeks after induction by 5-azacytidine and conditioned cultrure medium of cardiac muscle, the expression of myocardial troponin was measured by immunohistochemistry and analysed by one-way analysis of variance. RESULTS AND CONCLUSION: MSCs could be induced into myocardial cells by conditioned cultrure medium of cardiac muscle in vitro, indicating that the cytokines in the cultrure medium might play key roles in the induction. But such effect of conditioned cultrure medium of cardiac muscle was weaker than that of 5-azacytidine. The effect of combined induction was the strongest.