凝胶型E基质在大鼠脊柱后外侧融合中的应用

背景：重组人骨形态发生蛋白(recombinant human bone morphogenetic protein，rhBMP)的骨诱导能力已在各种动物模型和临床试验中得到验证。 目的：利用大鼠脊柱后外侧融合模型，分析凝胶型E基质作为rhBMP-2的新型载体在脊柱后外侧融合中的作用。 方法：建立大鼠后外侧融合模型，105只Lewis雄性大鼠在相同条件下分别在L4-5横突间植入可吸收明胶海绵复合不同质量的rhBMP-2，对加或不加凝胶型E基质进行分组观察。 结果及结论：10 μg和3 μg rhBMP-2两组融合率100%，1，0.5，0.1 μg rhBMP-2组的融合率分别是40%，10%，0%。加E基质的0.5 μg以上的rhBMP-2组全部融合，而0.1，0.05 μg rhBMP-2组未发现融合迹象。Micro-CT扫描提示3 μg rhBMP-2复合明胶海绵加E基质与10 μg rhBMP-2复合明胶海绵组融合节段形成的新生骨骨量相似，而0.1，0.05 μg rhBMP-2不管有无E基质，都无新骨形成。说明应用E基质作为载体能明显提高rhBMP-2在脊柱后外侧的融合成功率，但当rhBMP-2的剂量过小时，使用E基质做载体也不能达到预期的脊柱融合。

Application of glue-like E-matrix in posterolateral spinal fusion of rats

BACKGROUND:Various animal models and clinical trials show that the recombinant human bone morphogenetic protein (rhBMP) has the capacity of bone induction. OBJECTIVE:To study the effect of glue-like E-matrix as a new type of carrier for rhBMP-2 on posterolateral spinal fusion of rats. METHODS:Posterolateral fusion models were established in 105 male Lewis rats. Absorbable gelatin sponge and rhBMP-2 with different qualities were implanted into the L4-5 intertransverse of rats under the same conditions. Observation of adding glue-like E-matrix or not into groups was performed. RESULTS AND CONCLUSIONS:Fusion rates of the rats with 10 μg rhBMP-2 and 3 μg rhBMP-2 were 100%, and those of the rats with 1 μg, 0.5 μg and 0.1 μg of rhBMP-2 were 40%, 10% and 0% respectively. In the adding glue-like E-matrix groups, the fusion rate of rats with > 0.5 μg rhBMP-2 was 100%, but no fusion was observed in the rats with 0.1 μg and 0.05 μg rhBMP-2. The micro-CT results revealed that the amount of the new bone created in the 10 μg rhBMP-2 + E-matrix group and the 3 μg rhBMP-2+E-matrix group were similar. However, there was no new bone created in the 0.1 μg and 0.05 μg rhBMP-2 groups containing E-matrix or not. E-matrix can enhance the fusion rate of rhBMP-2 significantly in posterolateral spinal fusion. However, the expected fusion cannot be achieved by E-matrix if the dose of rhBMP-2 is too small.