转碱性成纤维细胞生长因子基因组织工程骨修复兔骨缺损

背景：生物活性玻璃是一类具有良好生物活性及骨修复特性的生物医用材料,将其与血、脱钙骨基质或与骨形态发生蛋白等混合来促进成骨形成,增加强度,骨愈合更接近于天然骨结构。目的：观察生物活性玻璃结合转碱性成纤维细胞生长因子基因骨髓间充质干细胞构建组织工程骨修复兔骨缺损的效果。 方法：以生物活性玻璃作为转碱性成纤维细胞生长因子基因骨髓间充质干细胞的可吸附载体,体外构建组织工程骨,将其植入兔桡骨中段10 mm骨缺损处,以自体骨移植组、生物活性玻璃/骨髓间充质干细胞组和空白组作为对照,术后2,4,8,12周进行影像学、病理组织切片、生物力学测试,观察各组骨缺损修复效果。 结果与结论：以生物活性玻璃作为转碱性成纤维细胞生长因子基因骨髓间充质干细胞的可吸附载体,体外构建的组织工程骨植入兔桡骨骨缺损的成骨效应、骨愈合后的抗扭转强度明显优于其他各组(P < 0.01)。说明生物活性玻璃结合转碱性成纤维细胞生长因子基因骨髓间充质干细胞构建的组织工程骨可用于骨缺损修复,优于自体骨移植。

Tissue engineered bone with transfected basic fibroblast growth factor for the repair of bone defects in rabbits

BACKGROUND:Bioactive glass is a kind of biomedical materials with good bioactivity and bone repair properties, its combination with blood, demineralized bone matrix or bone morphogenetic protein could promote bone formation and increase strength, bone healing is close to natural bone structure.OBJECTIVE:To investigate the efficacy of bioactive glass combined with transfected basic fibroblast growth factor (bFGF) and bone marrow mesenchymal stem cells (BMSCs) for repairing bony defects in rabbits. METHODS:Tissue engineering bone was in vitro prepared with bioactive glass serving as an adsorbable carrier for transfected bFGF and BMSCs. The tissue engineered bone was implanted into 10-mm bone defects in middle radius of adult rabbits, while bioglass/BMSCs, autograft implantation and blank control with no graft were used as controls. The animals were sacrificed on 2, 4, 8, 12 weeks after operation. The bone repair was investigated using imaging, histological slice and biomechanical test methods. RESULTS AND CONCLUSION:With bioactive glass serving as an adsorbable carrier for transfected bFGF and BMSCs, the tissue engineered bone constructed in vitro were implanted into radial defects of rabbits, the bone formation and anti-torsional strength after bone healing were significantly better than other groups (P < 0.01). Tissue engineering bone can efficiently repair bony defects, and is superior to autograft implantation.