异种脱蛋白松质骨支架体内移植后血清T细胞亚群的变化

背景：研究提示异种脱蛋白松质骨可能成为一种极有实用价值的骨组织工程支架材料，但仍缺少细胞生物学及免疫学论证。目的：脱蛋白法制备骨组织工程用的异种松质骨支架，探讨其山羊体内移植后免疫学性能。方法：成年猪股骨远端松质骨，采用逐级脱蛋白方法去除异种骨中的抗原性成分。取6~8月龄雄性山羊8只随机分成2组，实验组双侧3、4横突间各植入脱蛋白松质骨2块。对照组植入相同数量未经处理新鲜松质骨。结果与结论：采用脱蛋白处理后的猪松质骨光镜下可见骨孔内无细胞、神经、血管和嗜酸性物质染色。脱蛋白松质骨中胶原类氨基酸含量高，与新鲜松质骨相比无明显差异，而酪氨酸、色氨酸和半光氨酸波峰消失。新鲜松质骨氨基酸含量为19.89%，脱蛋白松质骨氨基酸含量为18.06%。脱蛋白松质骨植入后各时间点CD3⁺、CD4⁺、CD8⁺、CD28⁺水平与植入前相比基本保持一致，植入4周后局部抗原免疫组织化学未见阳性表达。提示经脱蛋白处理后异种松质骨低免疫原性，植入体内后没有特异性抗体产生，具有良好的组织相容性。

Immune properties of xenogeneic-deproteinized-cancellous-bone as tissue engineered scaffold

BACKGROUND:It has showed that deproteinized cancellous bone maintains natural network pore system and provides better cancellous compatibility than other mechanical materials except immunology. OBJECTIVE:To fabricate xenogeneic-deproteinized-cancellous-bone scaffold and to explore the intensity of immune reaction sparked by xenogeneic deproteinized cancellous bone implanted at goats. METHODS:We made deproteinized bone derived from pig femoral cancellous bone by physical and chemical treatments. Their morphological features, constitute components and mechanical properties were examined by scanning electron microscopy, X-rays diffraction analysis, mechanical experimental instrument and so on. Eight male goals were divided into experimental and control group according to transplant materials. Materials included xenogeneic deproteinized cancellous bone and fresh xenogeneic cancellous bone.RESULTS AND CONCLUSION:It showed that deproteinized cancellous bone maintained natural network pore system. The main organic material was collagenⅠ and inorganic was hydroxyapatite. It hardly has any antigen and it has good histocompatibility. The mechanical strength was no significant difference between deproteinized cancellous bone and fresh cancellous bone. In experimental group, T lymphocyte subsets CD3⁺, CD4⁺, CD8⁺and CD28⁺ in blood samples had no significant difference at different time point, and there was no positive stained reddish zones in the slides of xenogeneic deproteinized cancellous bone implanted for 4 weeks at intertransverse. Xenogeneic deproteinized cancellous bone may be safe in immunology.