丹参素对大鼠牙槽骨代谢的影响

背景：有研究表明胰岛素样生长因子Ⅰ降低是骨丢失的早期标志。 目的：测定大鼠牙槽骨组织中胰岛素样生长因子Ⅰ的活性,探讨丹参素防治牙槽骨骨质疏松症的可能机制。 方法：SD大鼠随机分为对照组、去卵巢组和丹参素组,后两组行双侧卵巢切除,对照组和去卵巢组用蒸馏水灌胃,丹参素组用丹参素12.5 mg/(kg•d)灌胃。给药90 d后取大鼠牙槽骨,检测牙槽骨量、牙槽骨组织中胰岛素样生长因子Ⅰ活性。 结果与结论：与对照组相比,去卵巢组骨小梁面积百分数减少33.88%(P < 0.05),骨小梁分离度增加43.58%(P < 0.05),牙槽骨骨量明显减少,牙槽骨组织中胰岛素样生长因子Ⅰ表达变少、变弱(P < 0.05)。与去卵巢组相比,丹参素组骨小梁面积百分数增加了45.45%(P < 0.05),牙槽骨骨量明显增多,牙槽骨组织中胰岛素样生长因子Ⅰ表达增强(P< 0.05)。结果证实,丹参素能促进牙槽骨组织胰岛素样生长因子Ⅰ活性,防止去卵巢大鼠牙槽骨的丢失。

Effects of tanshinol on alveoar bone metabolism in rats

BACKGROUND:Decrease in insulin-like growth factor 1 (IGF-1) is an early marker of bone loss. OBJECTIVE:To determine the activity of IGF-1 in rat alveoar bone tissue and investigate the possible mechanism by which tanshinol prevents alveolar bone osteoporosis. METHODS:Twenty-four Sprague-Dawley rats were randomly divided into three groups: sham-ovariectomized rats, ovariectomized rats and ovariectomized rats with tanshinol treatment (12.5 mg/kg per day). At 90 days after administration, alveolar bone tissue was taken for detection of bone mass and the activity of IGF-1 in alveolar bone tissue. RESULTS AND CONCLUSION:Compared with sham-ovariectomized rats, alveolar bone mass was decreased significantly, percentage of trabecular bone was decreased by 33.88% (P < 0.05), trabecular separation was increased by 43.58% (P < 0.05) and IGF-1 expression was markedly decreased (P < 0.05) in ovariectomized rats. Compared with ovariectomized rats, alveoar bone mass was increased significantly, percentage of trabecular bone was increased by 45.45% (P < 0.05) and IGF-1 expression was markedly increased (P < 0.05) in ovariectomized rats with tanshinol treatment. These findings confirm that tanshinol can increase the expression of IGF-1 in alveolar bone tissue and prevent alveoar bone osteoporosis.