酸性成纤维细胞因子复合部分脱蛋白骨修复早期股骨头缺血坏死的组织学改变

背景：以往研究显示，酸性成纤维细胞因子(acidic fibroblast growth factor，aFGF)复合部分脱蛋白骨(partially deproteinised bone，PDPB)对实验动物早期股骨头缺血坏死血管再生具有良好的促进作用，但其组织学变化尚不明确。目的：从组织学变化角度观察aFGF复合PDPB修复兔早期股骨头缺血坏死的效果。方法：用新西兰大白兔制备早期股骨头缺血坏死动物模型，建模后随机分为空白组、PDPB组和aFGF/PDPB组。PDPB组植入PDPB，aFGF/PDPB组植入aFGF/PDPB。所有动物分别于第2，4，8周取材进行苏木精伊红染色观察成骨情况。结果与结论：空白组第8周缺损区被纤维结缔组织填充，在交界处有少量骨样组织形成。PDPB组第4周有少量新骨生成，髓腔形成，第8周较多植入物吸收，髓腔形成，有很多成骨细胞及髓细胞分布其中。aFGF/PDPB组各时间点成骨都普遍优于PDPB组，4周组移植物腔填充以骨样组织，可见较多的成骨前体细胞和骨母细胞，可见较多的微血管，骨样组织开始重建。第8周植入物已被新骨取代，髓腔已形成具有很多骨髓细胞分布其中，骨小梁交界处有大量成骨细胞，同时有少量破骨细胞存在可能参与骨塑形，骨陷窝可见成熟骨细胞。组织学检测结果提示，在修复股骨头缺血坏死的效果上，aFGF复合PDPB优于单独应用PDPB。

Acidic fibroblast growth factor combined with partially deproteinized bone in repair of early-stage avascular necrosis of the femoral head in rabbits

BACKGROUND:Previous studies have demonstrated that acidic fibroblast growth factor (aFGF) combined with partially deproteinized bone (PDPB) (aFGF/PDPB) well promotes vascularization in animals with early-stage avascular necrosis of the femoral head (ANFH), but the histological results remain unknown.OBJECTIVE:To observe the histological repairing effects of aFGF/PDPB on early-stage ANFH in rabbits. METHODS:New Zealand rabbits were established models of bilateral ANFH and were randomly divided into a blank group, a simple PDPB group, and an aFGF/PDPB group. PDPB and aFGF/PDPB bone were implanted into the PDPB and aFGF/PDPB group accordingly. The blank group did not receive any implantation. At 2, 4, and 8 weeks after surgery, all animals were sacrificed for histological examination to observe the osteogenesis by hematoxylin-eosin staining.RESULTS AND CONCLUSION:Defects were filled with granulation tissues and fibrous connective tissues, only a little osteoid tissue formed at the borderline in the blank group at the end of the 8th week. In the PDPB group, a little new bone and cavitas medullaris formed. At 8 weeks, lots of graft was absorbed and cavitas medullaris formed with more osteoplasts and myeloid cells in it. The osteogenesis in the aFGF/PDPB group was better than that of PDPB group in each time point. At 4 weeks, the transplanted cavity was filled with osteoid tissues, a lot of osteogenic precursor cells and osteoblasts could be seen. Plenty of micrangium was observed, and osteoid tissues began to rebuild. At 8 weeks, the graft was replaced by bone tissues, and cavitas medullaris were formed with lots of bone marrow cells in it. At the borderline of the bone trabecula, there were lots of osteoplast and little osteoclasts, which may play a role in bone remodeling. There were mature bone cells in bone lacuna. Results indicate that aFGF/PDPB has better repair effect on rabbit model of ANFH than that of simple PDPB.