α-玉米赤霉醇干预骨质疏松性骨折模型大鼠白细胞介素1、6的表达

背景：α-玉米赤霉醇是一种新的植物雌激素，可能具有动物雌激素的某些生理功能。目的：观察植物雌激素α-玉米赤霉醇对骨质疏松性骨折大鼠白细胞介素1和白细胞介素6表达的影响。方法：切除40只6月龄雌性SD大鼠双侧卵巢建立去卵巢骨质疏松模型，造模成功后锯断右侧胫骨建立骨折模型，骨折1周，分别灌胃0.066 mg/kg雌二醇、10，5，1 mg/kg α-玉米赤霉醇及二甲基亚砜；另取10只大鼠，不切除卵巢，制备骨折模型后灌胃二甲基亚砜。给药35 d，ELISA法测定大鼠血清白细胞介素1和白细胞介素6的表达。结果与结论：去卵巢后，骨折大鼠血清白细胞介素1和白细胞介素6的表达明显升高，5，1 mg/kg α-玉米赤霉醇及0.066 mg/kg雌二醇均可明显降低骨质疏松性骨折大鼠血清白细胞介素1和白细胞介素6的表达，但10 mg/kg α-玉米赤霉醇作用效果不显著。说明α-玉米赤霉醇可以剂量依赖性地降低骨质疏松性骨折大鼠血清白细胞介素1和白细胞介素6的表达。

Effect of a-zearanol on interleukin-1, 6 expressions in a rat model of osteoporotic fracture

BACKGROUND:a-zearanol (α-ZAL) is a new phytoestrogen that may have some physiological functions of animal estrogen.OBJECTIVE:To investigate the effects of α-ZAL on interleukin (IL)-1and IL-6 in rats with osteoporotic fracture. METHODS:Osteoporosis was induced in 40 female 6-week-old Sprague-Dawley rats by bilateral ovariectomy. Thereafter, the right tibia was cut off to establish rat models of fracture. One week later, rats were intragastrically administered 0.066 mg/kg estradiol, or 10, 5, 1 mg/kg α-ZAL and dimethyl sulfoxide; another 10 rats did not underwent ovariectomy, but developed fracture and received intragastric administration of dimethyl sulfoxide. After administration for 35 days, serum level of IL-1 and IL-6 was determined by ELISA. RESULTS AND CONCLUSION:After ovariectomy, serum level of IL-1 and IL-6 was obviously increased in fracture rats. 5,  1 mg/kg α-ZAL and 0.066 mg/kg estradiol could markedly decrease serum level of IL-1 and IL-6 in osteoporotic fracture rats, but   10 mg/kg α-ZAL did not produce obvious effects. The results showed that α-ZAL could decrease serum level of IL-1 and IL-6 in osteoporotic fracture rats in a dose-dependent manner.