组织工程椎间盘纤维环的研究与进展

背景：组织工程是永久性修复退变椎间盘的新策略,然而髓核组织工程方法无法完整重建椎间盘的结构和功能,所以,相应的纤维环组织工程被认为是组织工程椎间盘治疗策略的主要限制因素之一。 目的：综述组织工程椎间盘纤维环的研究与进展,为组织工程椎间盘研究提供理论基础。 方法：由第一作者用计算机检索Pubmed、CNKI及维普数据库,限定时间为2000/2010,中英文检索词分别为“组织工程、椎间盘、纤维环、种子细胞、支架材料、生长因子”和“tissue engineering, intervertebral disc, annulus fibrosus, seed cell, scaffold, growth factor”,语言分别设定为中文和英文。从组织工程椎间盘纤维环的种子细胞来源,支架材料选择,生长因子3方面进行归纳介绍。 结果与结论：共检索到106篇文章,按纳入和排除标准对文献进行筛选,保留34篇文章进行综述。目前组织工程椎间盘研究重点在构建纤维环组织与髓核组织上。关于功能化纤维环的构建还处于起始阶段,种子细胞、支架材料以及生长因子的选取仍需进一步的实验研究。

Research and progress of tissue-engineered annulus fibrosus disci intervertebralis

BACKGROUND:Tissue engineering is a new strategy that can permanently repair degenerated intervertebral disc. However, nucleus pulposus tissue engineering method cannot completely reconstruct the structure and function of intervertebral discs. Therefore, the corresponding anulus fibrosus tissue engineering is regarded as one of the major limiting factors for the treatment strategies of tissue-engineered intervertebral disc. OBJECTIVE:To sum up the research and progress of tissue-engineered annulus fibrosus disci intervertebralis, and to provide theoretical foundation of tissue-engineered intervertebral disc. METHODS:Pubmed database, CNKI database, and VIP database from 2000 to 2010 was retrieved by the first author with the key words of “tissue engineering, intervertebral disc, annulus fibrosus, seed cell, scaffold, growth factor” in Chinese and in English, respectively. Literatures were limited to Chinese and English languages. Seed cell source, the selection of scaffold materials, and growth factor of annulus fibrosus disci intervertebralis tissue engineering were summarized. RESULTS AND CONCLUSION:A total of 106 literatures were obtained from computer screen, 34 of them were involved for summarization according to inclusion and exclusion criteria. At present the studies were concentrated on the construction of annulus fibrosus and nucleus pulposus tissues. And the construction of functional annulus fibrosus is still at the initial stage. The selection of the seed cells, scaffold materials and growth factors are still to be further studied.