嗅鞘细胞和神经干细胞联合移植阿尔茨海默病大鼠脑内的增殖和定向分化

背景：阿尔茨海默病大鼠脑内神经元减少,神经干细胞移植后增殖和向神经元分化能力有限。目的：观察联合移植嗅鞘细胞和神经干细胞在阿尔茨海默病大鼠脑内,嗅鞘细胞对神经干细胞的增殖和向胆碱能神经元分化的影响。方法：体外培养嗅鞘细胞和神经干细胞,移植前用5-溴脱氧尿嘧啶核苷标记神经干细胞。将生理盐水,神经干细胞和神经干细胞+嗅鞘细胞分别移植入阿尔茨海默病模型大鼠海马。移植7,14,21,28 d后,进行大鼠脑组织切片免疫组织化学染色检测BrdU和ChAT阳性表达。结果与结论：联合移植组神经干细胞的增殖和分化情况明显优于其他两组,联合移植组BrdU阳性细胞数和ChAT阳性细胞数明显高于神经干细胞移植组和生理盐水组(P < 0.01)。提示嗅鞘细胞能促进移植的神经干细胞在阿尔茨海默病大鼠脑内增殖和向胆碱能神经元的分化。

Proliferation and directed differentiation of neural stem cells and olfactory ensheathing cells after co-transplantation into the brain of Alzheimer’s disease rats

BACKGROUND:The number of neurons is reduced in the brain of Alzheimer’s disease rats, and the proliferation and differentiation ability of transplanted neural stem cells (NSCs) into neurons is limited.OBJECTIVE:To investigate the effects of olfactory ensheathing cells (OECs) on the proliferation and differentiation of NSCs to cholinergic neurons after co-transplantation into the brain of Alzheimer’s disease rats.METHODS:OECs and NSCs were separately cultured in vitro, and the NSCs labeled with 5-bromodeoxyuridine (BrdU) before transplantation. Normal saline, NSCs and NSCs+OECs were separately transplanted into the hippocampi of Alzheimer’s rats. At 7, 14, 21 and 28 days after transplantation, the immunohistochemical staining was used to detects the expression of BrdU and choline acetyltransferase (ChAT) in the slice of rat brain tissues to analyze the transplanted cells’ proliferation and differentiation.RESULTS AND CONCLUSION:The condition of proliferation and differentiation of the NSCs co-transplanted with OECs was the best. The BrdU-positive cells and ChAT-positive cells of the OECs and NSCs co-transplantation group were the most of the three groups (P < 0.01). OECs can promote the proliferation and differentiation of NSCs to cholinergic neurons after co-transplantation in the brain of Alzheimer’s disease rats.