MMP-9 -1562C/T基因多态性与2型糖尿病肾病的关系研究

摘要 目的：探讨2型糖尿病（T2DM）患者基质金属蛋白酶-9基因-1562C/T（MMP-9 -1562C/T）多态性与糖尿病肾病（DN）的关系以及与DN不同病期的相关性。方法：将150例T2DM患者按DN诊断标准分为DN组与非肾病（NDN）组，DN组分为微量白蛋白尿期、临床白蛋白尿期和肾功能不全期。另择52例健康人作为正常对照（NC）组。应用限制性片段长度多态性分析各组基因型。结果：DN组的HbA1c、SBP、Cr、BUN、UAER值高于NDN、NC组（P<0.01）。DN组分别和NDN组、NC组之间基因型分布差别均有统计学意义（P<0.01）。T2DM中CC、CT、TT各基因型DN发病率递减，分别为59.7%、56.7%、34.9%。C和T等位基因DN发病率分别为59.2%、40.5%，携T等位基因者发生DN的风险是携C等位基因者的0.47倍（P<0.01，95%CI 0.29～0.75）；DN组内3期TT基因型与T等位基因频率随肾功能降低而递减；结论：MMP-9 -1562C/T基因多态性与DN发生有关，T等位基因是DN患者的保护基因。

Study on the association of the matrix metalloproteinase-9 gene -1562C/T polymorphism with diabetic nephropathy

Abstract Objective: To explore the dependability of matrix metalloproteinase-9 (MMP-9) gene -1562C/T and diabetic nephropathy (DN), and the relationship with different stage of DN. Methods: 150 type 2 diabetes (T2DM) patients were divided into DN group and diabetes without DN (NDN) group. DN group included microamount albuminuria, clinical alubuminuria and renal inadequacy stage. 52 healthy people were selected as normal control (NC) group. The genotypes were assessed by restriction fragment length polymorphism．Results: The average HbA1c、SBP、Cr、BUN、UAER of DN group were significantly higher than that of NDN or NC group (P<0.01).There was significant difference in frequencies of allele or genotype of polymorphismC/T between three groups (P<0.01). DN incidence rate in type 2 diabetics containing with genotype of CC, CT or TT respectively were decreased gradually. DN incidence rate containing with allele C and T were 59.2% and 40.5% respectively. The T allele carriers had 0.47 times risk for increased incidence of DN compared with that of C allele carriers (P<0.01，95%CI 0.29～0.75). The frequencies of TT genotype and T allele in three stages of DN decrease with renal hypofunction. Conclusion: The polymorphism of MMP-9 -1562C/T is associated with the development of DN, T allele is the protective gene of DN.