鼠胚神经干细胞移植对帕金森模型大鼠的治疗作用

背景：干细胞移植是治疗帕金森的有潜力的方法之一。目的：观察神经干细胞纹状体移植对帕金森模型大鼠旋转行为及脑内多巴胺含量的影响。方法：采用6-羟基多巴胺定点注射毁损黑质纹状体的方法构建帕金森大鼠模型;向造模成功的大鼠纹状体内分别移植1×106(共计20 μL)的第3代胚鼠神经干细胞或等量生理盐水。结果与结论：神经干细胞移植后,帕金森大鼠的旋转行为明显改善。干细胞移植后3周,免疫组化检测发现移植干细胞的帕金森大鼠脑黑质部位酪氨酸羟化酶阳性细胞数增多,纹状体内可见酪氨酸羟化酶阳性细胞;荧光显微镜下观察发现Hoechst 33324d标记神经干细胞在移植针道附近最为密集,并向远隔部位迁徙。干细胞移植后8周,高效液相色谱检测显示移植干细胞的帕金森大鼠纹状体内多巴胺含量明显增高(P < 0.01)。说明神经干细胞脑内移植能够减轻6-羟基多巴胺引起的大鼠中脑黑质多巴胺能神经元的损伤,改善大鼠的旋转行为。

Effects of engrafted neural stem cells derived from fetal rat on model rat with Parkinson's diseases

BACKGROUND:Stem cells transplantation is one of the potential methods for the treatment of Parkinson's diseases (PD).OBJECTIVE:To investigate the effect of neural stem cells engrafted into the striatum on rotational behavior and dopamine concentration of rat model with PD. METHODS:Rat models of PD were established by point injection with 6-hydroxy dopamine to damage nigrostriatal, the 1×106 (total 20 μL) passage 3 fetal rat neural stem cells or normal saline were injected into the striatum of the established rat model.   RESULTS AND CONCLUSION:After neural stem cells were transplanted into the striatum, the rotational behavior of the PD rat was improved obviously. Three weeks later, the results of immunohistochemistry detection showed that the number of tyrosine hydroxylase-positive cells in the substantia nigra of transfected PD rat was increased obviously, and the tyrosine hydroxylase-positive cells could be seen in the striatum. Fluorescence microscope observation showed that the Hoechst 33324d marked stem cells were more intensive near the site of transplantation and migrated to distant part. Eight weeks later, high performance liquid chromatography analysis showed that dopamine concentration in the striatum of stem cells transplanted PD rats was significantly higher (P < 0.01). It indicates that intracerebral neural stem cells transplantation can reduce the rat substantia nigra dopaminergic neuronal damage caused by 6-hydroxy dopamine, and improve the rotational behavior of PD rat.