裸鼠皮下种植性肝癌模型的建立

背景：目前采用Huh7肝癌细胞在BALB/c裸小鼠皮下种植性成瘤的报道较多，但对其成瘤后稳定性的研究较少。 目的：建立稳定的皮下种植性裸鼠肝癌模型。 方法：取1.5×10⁶个对数期细胞Huh7肝癌细胞，种植于裸鼠皮下。成瘤后从体质量、大体形态，肿瘤生长情况，反转录-聚合酶链式反应，苏木精-伊红染色及免疫组织化学多方面进行稳定性及病理学特征鉴定。 结果与结论：肿瘤形成的潜伏期约12 d，肿瘤成功率为92.8%。种植肿瘤组小鼠体质量较正常对照小鼠明显减轻，肿瘤生长迅速，甲胎蛋白基因及蛋白强阳性表达，细胞分裂明显。表明成功建立了稳定的裸鼠皮下种植性肝癌模型。

Establishment of nude mouse models of subcutaneously implanted hepatocellular carcinoma

BACKGROUND:There have been more reports describing subcutaneous implantation of Huh7 hepatocellular carcinoma cells into BALB/c nude mice to induce hepatocellular carcinoma, but little is known about the stability after tumor formation. OBJECTIVE:To establish nude mouse models of stable subcutaneous hepatocellular carcinoma. METHODS:1.5×10⁶ Huh7 hepatocellular carcinoma cells were subcutaneously implanted into nude mice. After tumor formation, hepatocellular carcinoma stability and pathological characterization were performed in terms of body mass, gross anatomy, and tumor growth by reverse transplantation-polymerase chain reaction, hematoxylin-eosin staining, and immunohistochemical staining. RESULTS AND CONCLUSION:The latency of tumor formation was about 12 days, and success rate of tumor formation was 92.8%. In the tumor group, the body mass of nude mice was less compared with the control group, tumor developed rapidly, α-fetoprotein gene and protein expression was strongly positive, and hepatocellular carcinoma cells divided obviously. These findings suggest successful establishment of nude models of subcutaneously implanted hepatocellular carcinoma.