急性髓细胞性白血病患者外周血单个核细胞CD28 mRNA的表达

背景：大量研究显示,肿瘤患者外周血T细胞表面共刺激分子CD28蛋白表达存在差异,提示共刺激通路异常可能与恶性肿瘤的发生进展有关。 目的：观察急性髓细胞性白血病外周血单个核细胞共刺激信号分子CD28 mRNA在中的表达。 方法：急性髓细胞性白血病患者80例,其中M0型7例,M1型6例,M2型18例,M3型15例,M4型17例,M5型9例,M6型8例。并根据急性白血病疗效标准将80例患者分为完全治愈组、缓解组、未缓解组。采用Taqman探针实时荧光定量PCR检测80例患者及76名健康人群外周血单个核细胞CD28 mRNA的表达。 结果与结论：急性髓细胞性白血病外周血单个核细胞M1,M3和M4亚型中的CD28 mRNA表达量低于健康人群 (P< 0.05);急性髓细胞性白血病未缓解组中CD28 mRNA低于健康人群 (P < 0.05),完全治愈组和缓解组中CD28 mRNA表达与健康人群差异无显著性意义。说明急性髓细胞白血病患者外周血单个核细胞存在CD28 mRNA表达缺陷,并与临床分期、病情进展及预后有关。

CD28 mRNA expression in peripheral blood mononuclear cells of patients with acute myeloid leukemia

BACKGROUND:Recent studies have found that there is difference in costimulatory molecule CD28 protein expression on the surface of peripheral blood T cells in tumor patients, which suggested that abnormal costimulation pathway may be associated with the occurrence and progress of malignant tumor. OBJECTIVE:To investigate the costimulatory molecule CD28 mRNA expression in peripheral blood mononuclear cells of acute myeloid leukemia patients. METHODS:A total of 80 patients with acute myeloid leukenmia patients were selected, including 7 cases of M0 type, 6 cases of M1 type, 18 cases of M2 type, 15 cases of M3 type, 17 cases of M4 type, 9 cases of M5 type, and 8 cases of M6 type. According to acute leukemia curative effect criteria, 80 patients were assigned to complete healing group, remission group and non-remission group. Using Taqman probe, real-time fluorescent quantitation polymerase chain reaction was used to detect CD28 mRNA expression in peripheral blood mononuclear cells of 80 patients and 76 healthy persons. RESULTS AND CONCLUSION:CD28 mRNA expression was lower in the M1, M3, M4 subtype in peripheral blood mononuclear cells of patients with acute myelocytic leukemia compared with healthy population (P < 0.05). CD28 mRNA expression in non-remission group was lower than healthy population (P < 0.05). No significant difference in CD28 mRNA expression was detected between the complete healing and remission groups and healthy population. Results suggested that acute myeloid leukemia patients possess defects in peripheral blood mononuclear cells CD28 mRNA expression which associates with the clinical stage, disease progress and prognosis.