胶原-壳聚糖支架材料与间充质干细胞的组织相容性

背景：近年来一些研究发现胶原蛋白-壳聚糖复合支架材料可作为神经组织工程的支架材料，但相关细胞相容性研究较少。 目的：观察兔骨髓间充质干细胞在胶原蛋白-壳聚糖复合支架材料表面生长及分化情况。 方法：分离培养兔骨髓间充质干细胞，无血清培养液培养，流式细胞仪检查细胞表型；然后，将其接种到凝胶支架材料表面(实验组)及多聚赖氨酸包被的盖玻片表面(对照组)，神经诱导培养基内培养，倒置相差显微镜观察干细胞的生长及分化情况。 结果与结论：细胞表型为CD29⁺、CD44⁺、CD166⁺。倒置相差显微镜观察：实验组中，接种的骨髓间充质干细胞生长良好，7 d后可见有突起神经细胞，细胞生长情况与对照组未见有明显差别。证实胶原蛋白-壳聚糖复合支架材料对骨髓间充质干细胞有良好细胞相容性。

Histocompatibility of bone marrow mesenchymal stem cell and collagen-chitosan scaffold

BACKGROUND:Recent studies have showed that the collagen-chitosan complex scaffold can be used as nerve tissue engineering scaffold, but little evidence is available in the histocompatibility study. OBJECTIVE: Toobserve rabbit bone marrow mesenchymal stem cells (MSCs) growth and differentiation in the surface of collagen-chitosan complex scaffold. METHODS:Rabbit MSCs were isolated and cultured in serum-free medium. Flow cytometry was used to analyze the immunophenotype. The MSCs were planted on the gel scaffold (experimental group) and polylysine coated coverslip (control group), the cells were cultured in neural induction medium. The cell differentiation and growth characteristics were observed by inverted phase contrast microscope. RESULTS AND CONCLUSION:The phenotype was CD29⁺, CD44⁺, CD166⁺. Inverted phase contrast microscope showed that: the MSCs grew well with the prominence process seen at 7 days in experimental group. The cells growth characteristics had no difference with the control group. The MSCs and collagen-chitosan complex scaffold have good histocompatibility.