骨髓间充质干细胞移植并用单唾液酸神经节苷脂治疗大鼠脑梗死

背景：神经节苷脂是目前世界公认的惟一具有修复神经损伤，重塑神经网络的特效物质。单唾液酸神经节苷脂是神经节苷脂的一种。 目的：观察骨髓间充质干细胞移植并应用单唾液酸神经节苷脂治疗大鼠脑梗死的效果。 方法：Wistar大鼠应用线栓法建立大脑中动脉阻塞模型后随机分为3组，建模成功6 h后通过尾静脉注射浓度为1×1010 L-1的骨髓间充质干细胞悬液或细胞培养液1 mL，同时经腹腔注射单唾液酸神经节苷脂水溶液或生理盐水，1次/d，连续3 d。静脉移植后24 h，3 d及伤后1、2 周行Longa行为学评分，检测神经功能的损伤情况。治疗3 d后用RT-PCR、Western Blot检测脑组织中AQP4mRNA表达和蛋白合成的变化。2周后行BrdU免疫组化和苏木精-伊红染色观察移植细胞的存活和对组织的修复情况。 结果与结论：移植后1，2周，大鼠神经功能障碍评分细胞移植+单唾液酸神经节苷脂组低于细胞移植组，细胞移植组低于梗死组(P < 0.05)；移植后3 d，脑梗死周围组织AQP4蛋白及其mRNA的表达梗死组高于细胞移植组，细胞移植组高于细胞移植+单唾液酸神经节苷脂组(P < 0.05)；2周后BrdU免疫组化和苏木精-伊红染色切片中的神经元数量细胞移植+单唾液酸神经节苷脂组多于细胞移植组，细胞移植组多于梗死组(P < 0.05)。结果提示骨髓间充质干细胞移植的同时应用神经节苷脂治疗可明显改善脑梗死大鼠的神经学功能。

Monosialoganglioside combined with bone marrow mesenchymal stem cells transplantation for the treatment of cerebral infarction in rats

BACKGROUND:The ganglioside is the only world-recognized repair nerve damage, remodeling sovereign remedy of neural network. Monosialoganglioside (GM1) is one kind of ganglioside. OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation combined with GM1 on the treatment of cerebral infarction in rats. METHODS:The middle cerebral artery occlusion (MCAQ) models in Wistar rats were established by suture method, which were randomly divided into 3 groups: cell transplantation group, infarction group and cell transplantation + GM1 group. The concentration of 1×1010/L BMSCs suspension or the concentration of 1 mL cell culture fluid was injected into MCAQ models through caudal vein, at the same time, GM1 aqueous solution or normal saline was injected into MCAQ models through abdominal cavity, once a day, for 3 days. Longa ethology scores were determined at 24 hours, 3 days after vein transplantation, and 1, 2 weeks after MCAQ. Neurological damage was detected. After 3 days treatment, RT-PCR and Western Blot was used to detect changes of AQP4 mRNA expression and protein synthesis. After 2 weeks, the survival of implanted cells and the repair of tissue were observed by BrdU immunohistochemistry and hematoxylin-eosin staining. RESULTS AND CONCLUSION: Theneurological dysfunction scores in cell transplantation group + GM1 group were significantly lower than those in cell transplantation group and infarction group at 1 and 2 weeks after transplantation (P < 0.05). At 3 days after transplantation, AQP4 protein in the surrounding tissue cerebral infarction and its mRNA expression in infarction group were higher than those in cell transplantation and cell transplantation + GM1 groups (P < 0.05). The number of neurons in BrdU immunohistochemistry and hematoxylin-eosin staining slice in cell transplantation + GM1 group was higher than that in cell transplantation and infarction groups (P < 0.05). The results suggested that BMSCs transplantation combined with GM1 can significantly improve neurological function of cerebral infarction in rats.