肝细胞生长因子对过氧化氢诱导肝细胞凋亡的影响

背景：肝细胞生长因子对多种细胞具有保护作用。 目的：观察肝细胞生长因子对过氧化氢诱导肝细胞凋亡的保护作用及其机制。 方法：采用人LO2肝细胞系，随机分成3组：正常对照组为正常培养的LO2细胞；模型组加入100 mmol/L过氧化氢作用LO2细胞4 h；肝细胞生长因子组加入50 mg/L 肝细胞生长因子预处理LO2细胞24 h，再加入100 mmol/L过氧化氢继续培养4 h后处理细胞。 结果与结论：体外培养的LO2细胞经100 mmol/L过氧化氢作用4 h后，LO2细胞可出现明显的凋亡现象，表现为细胞存活率降低(P < 0.01)，Caspase-3蛋白表达增加(P < 0.01)，Bcl-2蛋白表达降低(P < 0.01)。给予质量浓度50 mg/L 肝细胞生长因子预处理24 h后再加入100 mmol/L过氧化氢继续培养4 h，LO2细胞的凋亡被显著抑制(P < 0.01)，说明肝细胞生长因子可通过增加LO2细胞Bcl-2的表达来抑制过氧化氢诱导的LO2细胞凋亡。

Effects of hepatocyte growth factors on H2O2-induced hepatocyte apoptosis

BACKGROUND:Hepatocyte growth factors exhibit protective effects on many cells.  OBJECTIVE:To investigate the protective effects of hepatocyte growth factors on H₂O₂-induced hepatocyte apoptosis of liver cells as well we the possible mechanism. METHODS:LO2 liver cell lines were randomly divided into three groups: normal control group: LO2 cells were conventionally cultured; model group: LO2 cells were treated with 100 mmol/L H₂O₂ for 4 hours; hepatocyte growth factor group: LO2 cells were pretreated with 50 mg/L hepatocyte growth factors for 24 hours and then further cultured with 100 mmol/L H₂O₂ for 4 hours. RESULTS AND CONCLUSION:After 4-hour treatment with 100 mmol/L H₂O₂, in vitro cultured LO2 cells showed obvious apoptosis, presenting with decreased cell survival rate (P < 0.01), increased caspase-3 protein expression (P < 0.01), and decreased Bcl-2 protein expression (P < 0.01). After 24-hour pretreatment with hepatocyte growth factors and subsequent 4-hour culture with 100 mmol/L H₂O₂, LO2 cell apoptosis was significantly inhibited (P < 0.01). These findings suggest that hepatocyte growth factors can inhibit H₂O₂-induced LO2 cell apoptosis by increasing Bcl-2 expression in LO2 cells.