鹿茸多肽干预膝骨性关节炎软骨细胞的增殖

背景：软骨细胞体外培养实验证实，鹿茸多肽其具有显著的促细胞有丝分裂活性，可刺激软骨细胞的增殖。 目的：观察鹿茸多肽对实验性骨性关节炎相关细胞因子的调节作用和对软骨细胞增殖的影响。 方法：将新西兰大白兔随机分成正常组，假手术组和模型组。正常组不予任何处理，假手术组左膝关节内侧皮肤切开后缝合，模型组建立左膝骨性关节炎模型。模型组建模成功后再将随机分为2组，鹿茸多肽组给予鹿茸多肽针剂生理盐水稀释液关节腔注射干预，生理盐水组给予生理盐水关节腔注射作为对照，干预后第1，7，15，30 天分别观察鹿茸多肽组和生理盐水组关节软骨形态学变化和软骨细胞结构变化；酶联免疫吸附法检测关节液中白细胞介素1β，肿瘤坏死因子α和转化生长因子β1的水平，免疫组织化学法检测关节软骨增殖细胞核抗原表达并计算细胞增殖指数。 结果与结论：在相同时间段内，与生理盐水组相比，鹿茸多肽组关节软骨增殖细胞核抗原表达，细胞增殖指数及关节液中转化生长因子β1含量均增高(P < 0.05)，关节液中白细胞介素1β和肿瘤坏死因子α水平明显降低 (P < 0.05)。结果证实鹿茸多肽可降低实验性骨性关节炎过程中白细胞介素1β和肿瘤坏死因子α水平，提高转化生长因子β1水平，并可促进关节软骨细胞的增殖。

Effect of pilose antler polypeptides on chondrocytes proliferation in knee osteoarthritis

BACKGROUND:Experimental studies on chondrocytes culture in vitro confirm that pilose antler polypeptide (PAP) has mitogenic activity and can stimulate the proliferation of cartilage cells. OBJECTIVE:To investigate the regulatory effect of PAP on related cytokines and chondrocytes proliferation in experimental osteoarthritis process. METHODS:Totally 80 New Zealand White rabbits were randomly divided into 3 groups: normal group (n=4), sham operation group (n=4) and model group (n=72).Normal group was not given any treatment. Sham operation group was sutured after incising the medial aspect of the left knee. And model group was surgically induced into osteoarthritis models. After successful modeling, the rabbits of the model group were further divided into 2 groups: PAP group and normal saline control group, 32 rabbits in each group. PAP group received 0.5 mL intra-articular injection of PAP dilution liquid once daily for 30 days while normal saline control group received 0.5 mL intra-articular injection of physiological saline. On days 1,7,15 and 30 after intervention, articular cartilage samples and synovial fluid were collected respectively. The morphological changes of articular cartilage were observed under optical microscope and the structural change of chondrocytes was observed by transmission electron microscopy. The levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and transforming growth factor β1 (TGF-β1 ) in synovial fluid were detected by enzyme-linked immunosorbent assay (ELISA). The expression levels of PCNA were detected by the method of immunohistochemistry and the cell proliferation index was analyzed. RESULTS AND CONCLUSION: At the same period, the levels of IL-1β and TNF-α in synovia fluid of PAP group were lower than those of normal saline control group, but the level of TGF-β1 was higher in the former group, which were statistically significant   (P < 0.05). The expression of proliferating cell nuclear antigen in artcular cartilage of PAP group was more than that of normal saline control group and the cell proliferation index was significantly higher, which had statistical difference. These results suggest that PAP can decrease the levels of IL-1β, TNF-α, increase the levels of TGF-β1 in synovial fluid and promote the proliferation of chondrocytes in experimental osteoarthritis process.