密度梯度离心法体外培养骨髓间充质干细胞的分化能力

背景：骨髓间充质干细胞具有多向分化能力，是目前极具潜力的种子细胞及基因治疗的靶细胞。目的：体外培养兔骨髓来源间充质干细胞，了解其生物学特性。方法：采用密度梯度离心法培养兔骨髓间充质干细胞，对细胞的形态及生长特性进行观察，应用流式细胞仪检测细胞表面抗原CD29、CD34、CD44、CD45、CD166、HLA-DR表达，并进行相关生物学特性鉴定。结果与结论：密度梯度离心法能分离培养出纯度较高的骨髓间充质干细胞，流式细胞仪检测骨髓间充质干细胞表达CD29、CD44、CD166，不表达CD34、CD45、HLA-DR。在适当的条件下能够诱导分化成为成骨细胞、软骨细胞等。

Differentiation ability of bone marrow mesenchymal stem cells cultured by density gradient centrifugation in vitro

BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) are capable of multiple directional differentiation, and are potential seed cells and target cells for gene therapy. OBJECTIVE:To observe in vitro cultured rabbit BMSCs, to understand its biological properties.METHODS:Density gradient centrifugation cultivation was utilized to culture rabbit BMSCs. The morphology and characteristics of cultured cells were observed. The surface markers CD29, CD34, CD44, CD45, CD166 and HLA-DR of cells were identified by flow cytometry, and its biological properties were identified. RESULTS AND CONCLUSION:High purity of rabbit BMSCs could be achieved by the density gradient centrifugation. The expressions of cell phenotypes were shown as follows respectively: CD29+, CD34-, CD44+, CD45-, CD166+, HLA-DR-. Under appropriate conditions, BMSCs were induced to differentiate into osteoblasts and cartilage cells.