用于骨力生物学研究的加载装置

背景：关节软骨组织细胞对力学刺激产生反馈来维持它的形态和结构,进而适应环境。加载装置能提供研究关节软骨力生物学的合适的力学环境。 目的：根据组织工程仿生的原理,建立一种用于软骨力学生物学研究的双频加载装置。 方法：该力学环境采用双频加载方式实现,通过控制可调凸轮和压电陶瓷振幅和频率来实现低频-高幅载荷耦合高频-低幅载荷,同时使用有限元法对受载体进行力学分析。 结果与结论：按照仿真的原理,研制出用于软骨力生物学研究的加载装置。在双频加载的条件下,软骨浅表层受到的力学载荷最大,其次是中间层,深层受到的力学应力最小;在高频10 Hz和20 Hz与低频1 Hz和2 Hz叠加时,软骨表现出不同的力学响应,但是二者引起的差异很小。该力学环境的构建可能有助于组织工程的发展和临床医学的应用,未来将采用生物学实验进行检验。

A loading device for study of bone mechanobiology

BACKGROUND:Articular cartilage cells respond to mechanical stimulation to maintain the morphological structure near cartilage of human body. A loading device can provide mechanical environment for studying mechanobiology.  OBJECTIVE: To construct a dual-frequency loading device according to the bionic principle of tissue engineering. METHODS:This mechanical environment was achieved by using dual-frequency loading method and implementing the coupling of low-frequency high-amplitude mechanical loading and high-frequency low-amplitude mechanical loading by controlling amplitude and frequency of cam and piezoelectric ceramics respectively, meanwhile, finite element method was used to make mechanics analysis on cartilage. RESULTS AND CONCLUSION:In the light of the principle of simulation, a dual-frequency loading device has been developed for bone mechanobiology study. Under dual-frequency loading condition, mechanical loading on the surface layer of cartilage was largest, followed by the middle layer, smallest on the deep layer; when high-frequency of 10 and 20 Hz superpositioning low-frequency of 1 and 2 Hz, the cartilage show different mechanical responses; but the difference between two response was little. The construction of the mechanical environment of the device may be helpful to tissue engineering development and clinical applications; in addition, it will be tested with biological experiment in future.