壳聚糖支架上骨髓间充质干细胞的浓度及黏附生长

背景：组织工程的研究重点是利用少量的细胞经体外培养、扩增后, 在一定环境下附着在三维多孔支架上并良好生长为后期的组织器官重建修复做好基础。目的：对不同浓度兔骨髓间充质干细胞复合至壳聚糖支架用于组织工程再生修复进行评价。方法：取5×10⁵脱乙酰度为95%的壳聚糖粉末通过冷冻干燥法制备壳聚糖支架,取1×10⁶,1×10⁷,1×10⁸,1×10⁹ L^-1细胞体积各100 μL复合至壳聚糖支架后1,3,5,7,9 d以光镜,扫描电镜,MTT法观察骨髓间充质干细胞的生长与分裂增殖情况。结果与结论：壳聚糖海绵状多孔支架为5 mm×5 mm×3 mm,孔径190~380 μm,平均孔径290 μm,孔相通性较好,空隙率为(84.00±4.62)%。细胞/支架共培养72 h后各浓度细胞组均可渗入壳聚糖支架多孔结构内黏附生长。1×10⁷,1×10⁸,1× 10⁹ L^-1浓度细胞组在支架上成蔟生长,部分细胞与支架融合。结果提示,1×10⁷,1×10⁸L^-1组细胞更利于骨髓间充质干细胞在壳聚糖支架的黏附生长,用于组织再生修复。

Concentration and adhesion of bone marrow mesenchymal stem cells on chitosan scaffolds

BACKGROUND:Tissue engineering research focuses on the use of a small number of cells cultured and amplified in vitro, in certain circumstances attached to the three-dimensional porous scaffolds and in a good growth for the reconstruction and repair of tissues and organs.OBJECTIVE:To evaluate the different concentrations of rabbit bone marrow mesenchymal stem cells (BMSCs) compounded on the chitosan scaffolds for tissue engineered regeneration and repair.METHODS:Chitosan scaffold was prepared by freeze-drying with 5×10⁵, 95% degree of deacetylation of chitosan powder. BMSCs growth and division were observed by optical microscope, scanning electron microscopy, and MTT method with 1×10⁶, 1×10⁷, 1×108, 1×10⁹/L cells of 100 μL compound on the chitosan scaffold at 1, 3, 5, 7, 9 days.RESULTS AND CONCLUSION:Sponge-like chitosan porous scaffold was 5 mm×5 mm×3 mm, the aperture was 190-380 μm, average aperture was 290 μm, the permeability was good, and the porosity was (84.00±4.62) %. At 72 hours after cell/scaffold co-culture, cells infiltrated the porous structure of chitosan scaffold and adhered to it. Cells of each group at concentration of 1×10⁷, 1×10⁸, 1×10⁹/L grew in cluster on the scaffold, some cells fused with the scaffold. It is indicated that 1×10⁷, 1×10⁸/L concentration of cells is more conductive to the adhesion growth of BMSCs on chitosan scaffold for tissue regeneration.