中药诱导骨髓间充质干细胞的成骨分化

背景：某些中药可以诱导骨髓间充质干细胞的成骨分化。骨髓间质干细胞向成骨分化的潜能，与中药治疗骨质疏松症、骨折、骨坏死、骨缺损等骨相关疾病有着理论上的相通性。目的：了解中药诱导骨髓间充质干细胞成骨分化的发展现状，为进一步的研究奠定基础。方法：由第一作者检索2000-01/2010-06中国期刊全文数据库(CNKI)(http://www.cnki.net/)及Pubmed数据库(http://www.ncbi.nlm.nih.gov/PubMed)。中文检索词为“中药，骨髓间充质干细胞，成骨分化”。英文检索词为“chinese herb, mesenchymal stem cells, osteogenic differentiation”。文献检索语种限定为中文和英文，纳入中药单体、单味中药、中药复方等及其含药血清在体内或体外对人或动物骨髓间充质干细胞成骨分化作用的研究文献，排除重复研究。结果与结论：共纳入32篇文献，有关骨髓间充质干细胞研究背景的文献2篇；有关单味中药的文献5篇，其中补肾药4篇，补气药1篇；有关中药复方的文献10篇，其中补肾方6篇，补肾活血方4篇；有关中药有效组分的文献15篇。补肾、补气及活血类中药可以诱导骨髓间充质干细胞向成骨细胞分化，但以补肾类中药为主，在一定程度上阐释了“肾主骨”理论的科学内涵，并为体外大量扩增骨髓间充质干细胞、促成骨分化及组织工程骨提供了更多的种子细胞来源。

Chinese herbs for inducing osteogenic differentiation of bone marrow mesenchymal stem cells

BACKGROUND:Some Chinese herbs can induce osteogenic differentiation of mesenchymal stem cells (MSCs). The potency of MSCs differentiate into osteogenesis has a theoretical communication with traditional Chinese medicine (TCM) treatment of osteoporosis, fractures, bone necrosis, bone defects and other bone-related diseases.OBJECTIVE:To understand the researching status of osteogenic differentiation of MSCs induced by Chinese herbs so as to establish further researching foundation. METHODS:CNKI database (2000-01/2010-06) and PubMed (2000-01/2010-06) were retrieved by the first author, with the key words of “Chinese herb, mesenchymal stem cells, osteogenic differentiation” in Chinese and in English. The literatures were limited to Chinese and English languages. Literatures concerning TCM monomer, single Chinese herb, Chinese herbal compound, and serum containing Chinese herb inducing osteogenic differentiation of MSCs in vivo and in vitro, human and animal were included. Repetitive studies were excluded. RESULTS AND CONCLUSION:A total of 32 literatures were included, including 2 documents addressing research background of MSCs, 5 single Chinese herbs (4 kidney-nourished herbs and 1 Qi-nourished herb), 10 Chinese herbal compound (6 kidney-nourished formulas and 4 kidney-nourished and activating blood formulas), 15 effective components of Chinese medicine. The kidney-nourished Chinese herbs, Qi- nourished herbs and activating blood herbs can induce MSCs into osteogenic differentiation, but the kidney-nourished Chinese herbs are majority. It explores the scientific value of the theory of “kidney control bone” and supply more resources of seed cells for a large number of amplification of MSCs in vitro, osteogenic differentiation and bone tissue engineering.