一种效果确切的大鼠重症急性胰腺炎模型

背景：目前尚无任何一种重症急性胰腺炎动物模型能与人类重症急性胰腺炎的发病过程完全一致,而用于实验治疗性研究的模型更是少之又少。 目的：建立一种大鼠重症急性胰腺炎模型,为进行大鼠重症急性胰腺炎的治疗性研究提供前提条件。 方法：30只SD大鼠随机分为对照组和模型组,对照组不建模,模型组大鼠采用逆行胰管穿刺注射法建立重症急性胰腺炎模型,建模后6,12,24 h,测血、腹水淀粉酶、血白细胞数量、血清白细胞介素6、肿瘤坏死因子α表达,并对胰腺组织进行病理评分。 结果与结论：模型组大鼠存活率80%,对照组大鼠存活率100%;模型组大鼠随时间的延长,病理评分、血、腹水淀粉酶、白细胞、白细胞介素6、肿瘤坏死因子α逐渐升高(P < 0.05)。说明逆行胰管注射法能够成功建立大鼠重症急性胰腺炎模型。

An effective rat model of severe acute pancreatitis

BACKGROUND:There is no animal model of severe acute pancreatitis that has the pathogenesis as same as human. Moreover, there are even fewer models used in experimental treatment studies. OBJECTIVE:To construct an effective rat model of severe acute pancreatitis for the therapeutic study on severe acute pancreatitis rats. METHODS:Thirty rats were randomly divided into control and model groups. The severe acute pancreatitis model was constructed by retrograde pancreatic duct infusion of Na-taurocholate. The expression of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α)  in the serum as well as amylase in the ascites and blood and white cell number in the blood were detected, and the pathological changes of pancreatic tissues were observed. RESULTS AND CONCLUSION:The survival rate was 80% in the model group, and 100% in the control group. With increasing time, pathological score, IL-6 and TNF-α expression in the serum, white cell number and amylase in the ascites and blood were increased gradually (P < 0.05). It was indicated that the severe acute pancreatitis model was constructed successfully in rats.