椎间注射转化生长因子β1对兔退变腰椎间盘蛋白多糖表达的影响

背景：体外研究证实转化生长因子β1可以促进蛋白多糖合成,延缓椎间盘退变,但体内实验鲜见报道。 目的：观察局部应用转化生长因子β1对兔退变腰椎间盘髓核蛋白多糖表达的影响。 方法：取30只新西兰大白兔建立兔腰椎间盘退变模型,造模12周,经X射线证实退变后,随机选择6只模型兔及6只未造模正常兔,处死取材。分别向剩余24只模型兔L_4~5椎间隙注射转化生子因子β1和生理盐水。末次给药2,4周取材,间苯三酚法测定兔椎间盘髓核内蛋白多糖的水平。 结果与结论：造模12周,兔退变椎间盘髓核中蛋白多糖水平明显降低(P < 0.01)。经局部注射转化生子因子β1后,兔退变椎间盘髓核中蛋白多糖表达明显增多(P < 0.01)。说明在兔椎间注射转化生子因子β1 可以促进髓核蛋白多糖的合成,延缓椎间盘退变。

Effects of intervertebral injection of transforming growth factor beta 1 on proteoglycan expression in rabbit degenerated lumbar discs

BACKGROUND:In vitro experiments have confirmed that transforming growth factor-β1 (TGF-β1) can promote proteoglycan synthesis and delay intervertebral disc degeneration, but its in vivo role remains unclear.  OBJECTIVE:To study the effect of local utilization of TGF-β1 on proteoglycan expression in rabbit degenerated lumbar discs. METHODS:Thirty-six New Zealand white rabbits were divided into two groups randomly, with 6 in control group and 30 in model group. The lumbar structure was destroyed partly in the model group. After 12 weeks, the intervertebral disc degeneration was testified by X-ray in model group. Six rabbits chosen randomly from the model group and all rabbits in the control group were killed and the organizations of the intervertebral discs of L_4-5 were harvested. Meanwhile, the rest rabbits in model group were divided into two groups randomly, which was injected with TGF-β1 saline. The rabbits were killed by 2 and 4 weeks after injection. The contents of proteoglycan in nucleus pulposus were tested by phloroglucinol method.  RESULTS AND CONCLUSION:The content of proteoglycan in the nucleus pulposus was obviously decreased at 12 weeks after model preparation (P < 0.01). After TGF-β1 injection, the content of proteoglycan was notably increased (P < 0.01). It is suggested that intervertebral injection of TGF-β1 to rabbit can delay intervertebral disc degeneration and enhance the synthesis of proteoglycan in nucleus pulposus.