急性脊髓损伤模型大鼠与白细胞介素1受体拮抗剂的保护作用

背景：白细胞介素1受体拮抗剂对大鼠急性脊髓损伤后脊髓功能修复具有保护作用,但具体机制不明。 目的：观察白细胞介素1受体拮抗剂对大鼠急性脊髓损伤组织神经丝蛋白质200和胶质纤维酸性蛋白的影响。 方法：SD大鼠随机分成假手术组,生理盐水对照组和白细胞介素1受体拮抗剂治疗组。采用改良Allen氏打击法建立急性脊髓损伤大鼠模型。分别在建模后1,48和72 h获取损伤段8 mm脊髓标本。 结果和结论：免疫组织化学染色检测,白细胞介素1受体拮抗剂治疗组神经丝蛋白质200和胶质纤维酸性蛋白的表达较假手术组和生理盐水组高,差异有显著性意义(P < 0.05)。提示白细胞介素1受体拮抗剂可使急性脊髓损伤大鼠模型损伤段脊髓神经丝蛋白质200和胶质纤维酸性蛋白表达增加,对急性脊髓损伤发挥保护作用。

Effect of interleukin-1 receptor antagonist on neurofilament protein 200 and glial fibrillary acidic protein expression in a rat model of acute spinal cord injury

BACKGROUND: Interleukin-1 receptor shows protective effects on spinal cord function recovery in rats with acute spinal cord injury, but the precise mechanism remains poorly understood. OBJECTIVE:To investigate the effect of interleukin-1 receptor antagonist (IL-1Ra) on neurofilament protein 200 (NF 200) and glial fibrillary acidic protein (GFAP) expression in rat acute spinal cord injury tissue. METHODS:A total of 90 Sprague-Dawley rats were randomly divided into three groups: sham-operated, normal saline and IL-1Ra treatment group. Acute spinal cord injury models were established by improved Allen’s method. At 1, 48 and 72 hours after model establishment, an 8-mm spinal cord segment was dissected longitudinally from the lesion site in each group. The NF200 and GFAP expression was detected by immunohistochemical staining. RESULTS AND CONCLUSION: NF200 and GFAP expression in the IL-1Ra trentment group was significantly higher than that in the sham-operated and normal saline group (P < 0.05). These results showed that IL-1Ra can increase NF200 and GFAP expression in the injured spinal cord segment and exhibit protective effect on acute spinal cord injury.