糖尿病小鼠理想的胰岛移植部位

背景：胰岛移植到糖尿病小鼠不同部位影响胰岛移植成功率。目的：比较糖尿病小鼠小网膜、肾被膜和腋窝3个部位胰岛移植效果,探索一个更理想的移植部位。方法：应用淋巴细胞分离液分离纯化BALB/c小鼠胰岛;将胰岛移植到造模成功的糖尿病C57BL/6小鼠,按照不同的移植部位小网膜、肾被膜和腋窝进行对比观察。结果与结论：分离纯化后获得高纯度胰岛,每只供体可获得胰岛(102±4)个,并具有良好生物活性;胰岛移植到糖尿病小鼠小网膜、肾被膜和腋窝3个部位后,均能降低血糖至正常范围,移植到小网膜与肾被膜的血糖值差异无显著性意义(P > 0.05),但均低于腋窝血糖值(P < 0.05)。移植后第7天,苏木精-伊红染色见小网膜部位移植胰岛形态基本完整,肾被膜部位移植胰岛形态不规则,腋窝部位移植物胰岛完全被破坏,伴炎症细胞浸润,小网膜和肾被膜降血糖效果优于腋窝。说明小网膜血供充足容量大,能够接纳较大体积的移植物,可能作为胰岛移植的理想部位。

Ideal islet transplantation sites in diabetic mice

BACKGROUND:Different islet transplantation sites in diabetic mice influence the success rate of islet transplantation. OBJECTIVE:To explore a more ideal transplantation site by comparing the transplantation effect of lesser omentum, kidney capsule and axillary fossa. METHODS:Lymphocytes separation medium was used to isolate and purify BACL/c mice islets, which were transplanted into C57BL/6 diabetic mice. Then the effect of different transplantation sites including lesser omentum, kidney capsule and axillary fossa was compared.RESULTS AND CONCLUSION:After isolation and purification, about (102 ± 4) islets per mouse were obtained and possessed excellent activity. After islets were transplanted into lesser omentum, kidney capsule and axillary fossa, the blood glucose of diabetic mice was significantly lower than normal extent (< 14.00 mmol/L). The blood glucose had no significant difference between lesser omental and kidney capsule groups (P > 0.05), but was significantly lower compared with axillary fossa group (P < 0.05). At 7 days after islet transplantation, the islet shape of lesser omentum group was basically complete, the islet shape of the kidney capsule group was irregular, that of the axillary fossa group was destroyed and many inflammatory cells were found in the axillary fossa group. The lesser omentum and kidney capsule groups regulated blood glucose level better than the axillary fossa group. The lesser omentum possessed larger capacity and better blood supply. The lesser omentum can be used as an ideal site of islet transplantation.