脂肪基质干细胞和骨髓基质干细胞的体外分化能力

背景：脂肪基质干细胞和骨髓基质干细胞具有很多相似的生物学特性。 目的：比较脂肪基质干细胞和骨髓基质干细胞与受损PC12细胞分别共培养后定向分化能力的差异。 方法：分别分离培养脂肪组织来源和骨髓组织来源的基质干细胞，取第5代细胞进行实验，2种细胞分别与正常或受损PC12细胞培养上清液共培养，或仅单独培养。 结果与结论：脂肪基质干细胞和骨髓基质干细胞均表达较高水平的CD44和CD29，而后者表达的CD45、CD56在前者几乎未检测到。单独培养的2种细胞均表达较高水平的Nanog、Oct4、Sox2，不表达神经元特异性烯醇酶。其中经受损PC12细胞干预的2种细胞Nanog、Oct4、Sox2表达水平显著降低，而脂肪基质干细胞中神经元特异性烯醇酶阳性细胞数更多，提示受损PC12细胞对于脂肪基质干细胞可能具有更强的诱导分化作用。

In vitro differentiation capacity of adipose-derived stromal stem cells and bone marrow stromal stem cells

BACKGROUND:Adipose-derived stromal stem cells (ADSCs) have many biological properties similar to bone marrow stromal stem cells (BMSCs). OBJECTIVE:To compare the directed differentiation of ADSCs and BMSCs co-cultured with damaged PC12 cells. METHODS:Stromal stem cells were isolated from adipose tissue and bone marrow. The fifth passage cells were selected. Two kinds of cells were co-cultured, respectively, with or without normal or damaged PC12 cells supernatant. RESULTS AND CONCLUSION:CD44 and CD29 highly expressed in ADSCs and BMSCs, while CD45 and CD56 were only detected in BMSCs, but rare in ADSCs. After single culture, two kinds of cells highly expressed Nanog, Oct4, Sox2, but not neuron-specific enolase (NSE). The levels of Nanog, Oct4, Sox2 in the cells co-cultured with damaged PC12 cells were significantly lowered, while NSE positive cells were increased in ADSCs. These indicate that damaged PC12 cells may have a stronger effect on induce and differentiation of ADSCs.