幽门螺杆菌全菌蛋白抗原壳聚糖微球体外释放特性

背景：不同方法制备出的幽门螺杆菌全菌蛋白抗原壳聚糖微球,其包裹率和控释效果也不同。目的：探讨幽门螺杆菌全菌蛋白抗原壳聚糖微球的最佳制备方案,观察其体外释放特性。方法：使用Berthold沉淀法制备壳聚糖微球,筛选最佳制备方案;使用扫描电镜及粒径分析仪观察壳聚糖微球的形态及粒径分布;冻干后的壳聚糖微球包裹幽门螺杆菌全菌蛋白抗原,使用BCA蛋白定量试剂盒测量分析微球的抗原包裹率、包裹量及释放率。结果与结论：从32种壳聚糖微球制备方案中筛选出了以海得贝壳聚糖为原料、冰乙酸的浓度为1%、硫酸钠为沉淀剂、pH值为5.0、不进行超声处理方案为最佳制备方案,扫描电镜示微球光滑圆整、致密,粒径分布在1.0~5.0 μm;抗原包裹率为79.92%,包裹量为16.47%;体外释放实验表明,总抗原释放率为20.39%,呈缓慢释放状态。

Preparation and in vitro release characteristics of chitosan microspheres loading Helicobacter pylori whole cell protein antigen

BACKGROUND:Chitosan microspheres loading Helicobacter pylori whole cell protein antigen prepared by different methods have different the package rates and controlled-release effects.OBJECTIVE:To prepare chitosan microspheres loading Helicobacter pylori whole cell protein antigen and observe the release characteristics in vitro. METHODS:Berthold precipitation method was used to prepare chitosan microspheres and the preparation was optimized; scanning electron microscope and particle size analyzer were used to observe the shape and size measurement of chitosan microspheres; the BCA Protein Assay Kit was used to measure the loading efficiency, loading capacity and releasing rate of microspheres. RESULTS AND CONCLUSION:We chose the best preparation program of chitosan microspheres from 32 species preparation programs: the best preparation program was the material of sea shell chitosan, 1% concentration of acetic acid, sodium sulfate for precipitating agent and 5.0 pH value, non-use of ultrasonic treatment. SEM results showed that the microspheres presented with smooth round and dense, diameters ranged from 1.0 μm to 5.0 μm; Antigen loading efficiency was 79.92%, loading capacity was 16.47%; Release experiment in vitro showed that the total antigen releasing rate was 20.39%, showing a slow-release status.