外周血间充质干细胞移植心肌梗死兔新生血管及心功能的变化

背景：药物治疗和支架置入治疗尚不能修复心肌梗死后已坏死的心肌。 目的：观察外周血间充质干细胞移植治疗对心肌梗死兔新生血管及心功能的影响。 方法：随机抽签法将36只大白兔分为假手术组,间充质干细胞移植组和对照组,结扎兔冠状动脉左室支建立心肌梗死模型。 结果与结论：移植后4周,流式细胞仪分析显示绝大部分间充质干细胞表达CD44,极少量细胞表达CD34和CD45。间充质干细胞移植组梗死心肌组织有移植的间充质干细胞存活,超声心动仪示间充质干细胞移植组左心室射血分数及短轴缩短率明显高于对照组(P < 0.01);左心室收缩末内径和舒张末内径明显小于对照组(P < 0.01)。间充质干细胞移植组心肌纤维化程度、心肌梗死面积均明显小于对照组(P < 0.01)。免疫组织化学染色显示间充质干细胞移植组新生毛细血管密度明显高于对照组(P < 0.01)。提示外周血间充质干细胞移植增加了梗死心肌新生血管密度,改善心脏的功能。

Effect of peripheral blood mesenchymal stem cells transplantation on neovascularization and cardiac function of myocardial infarction rabbits

BACKGROUND: Both Drug treatment and percutaneous coronary intervention cannot repair the necrotic myocardium following myocardial infarction. OBJECTIVE:To investigate the effect of peripheral blood mesenchymal stem cells (MSCs) transplantation on the neovascularization and cardiac function following myocardial infarction in rabbits. METHODS:Thirty-six New Zealand rabbits were divided into MSCs transplantation group (n=12), control group (n=12) and sham operation group (n=12). Rabbits models of acute myocardial infarction were established in the former two groups. RESULTS AND CONCLUSION:Flow cytometry demonstrated that most MSCs expressed CD44, very few cells expressed CD34 and CD45 at 4 weeks after cell transplantation. Transplanted MSCs could survive in infarcted myocardium. Left ventricular ejection fraction and left ventricular fractional shortening of the MSCs transplantation group were higher significantly than those of the control group (P < 0.01). Left ventricular end-systolic diameter and end-diastolic diameter were lower significantly in the MSCs transplantation group than the control group (P < 0.01). Myocardial fibrosis and myocardial infarct size were lower significantly in the MSCs transplantation group than the control group (P < 0.01). Capillary density was increased significantly in the MSCs transplantation group compared with the control group (P< 0.01). Peripheral blood MSCs transplantation increases new capillary density in infarcted myocardium and improves cardiac function.