不同来源骨髓间充质干细胞的体内成软骨

背景：骨髓间充质干细胞经体外诱导后可修复软骨缺损,但目前采用的种子细胞多来源于自体或同种异体。 目的：观察同种异体及异种来源的骨髓间充质干细胞诱导成软骨后修复喉软骨缺损的效果。 方法：分别取人胚胎骨髓间充质干细胞和刚出生兔骨髓间充质干细胞的第3代细胞种植于聚乳酸-羟基乙酸共聚物生物支架上,并加入转化生长因子β1和软骨形态发生蛋白诱导成软骨细胞。将两种细胞体系植入新西兰白兔体内,并于植入后4,8周取材行大体、组织学观察。 结果与结论：植入后4,8周人胚胎骨髓间充质干细胞和兔骨髓间充质干细胞均有新生组织填充,经组织学观察大部分为软骨细胞,分泌软骨细胞基质糖胺聚糖和Ⅱ型胶原,且两种细胞支架复合物所生成的软骨细胞数大致相同,并无明显的免疫排斥反应。提示异种来源的骨髓间充质干细胞复合聚乳酸-羟基乙酸共聚物在转化生长因子β1和软骨形态发生蛋白联合诱导下所得的组织工程化软骨,与同种来源的骨髓间充质干细胞所获得的组织工程化软骨修复喉软骨缺损具有可比性。

Chondrometaplasia of bone marrow mesenchymal stem cells of different resources in vivo

BACKGROUND:Bone marrow mesenchymal stem cells (BMSCs) can repair cartilage defects after induction in vitro. However, most of the seed cells come from autos or allograft currently. OBJECTIVE:To observe the effects of the chondrometaplasia of homologous and heterogeneous BMSCs on repairing the laryngeal cartilage defects. METHODS:The third generation of human embryonic BMSCs and rabbit BMSCs were involved. The two kinds of cells grew on poly lactic acid-glycolic acid scaffold, and were inducted to cartilage cells after adding transforming growth factor β1 and cartilage morphogenetic proteins into the scaffold. The two kinds of cell systems were implanted in New Zealand rabbits. The defects were removed at 4 and 8 weeks after implantation for general and histological observation. RESULTS AND CONCLUSION:The human embryonic BMSCs and rabbit BMSCs were filled with regenerated tissues at 4 and 8 weeks after implantation. Histological observation showed that most cells in the tissues were cartilage cells which could secrete glycosaminoglycan and collagen Ⅱ. The two types of cell scaffold could generate cartilage cells in about the same amount, and there was no immunological rejection between them. Results revealed that the chondrometaplasia of the heterogeneous BMSCs induced by transforming growth factor β1 and cartilage morphogenetic proteins is similar to the homologous BMSCs for repair of the laryngeal cartilage defects.