血管内皮生长因子及其受体在大鼠附睾内精子上的表达 |
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作者姓名: | 艾庆燕 赵豫凤 王艳梅 苗乃周 马 莉 杨加周 |
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作者单位: | 延安大学医学院组织胚胎学教研室,陕西省延安市 716000 |
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基金项目: | 陕西省科技厅自然科学基础项目(2010JM4024);陕西省教育厅专项科研计划项目(2010JK906,2010JK907);延安市科学技术研究发展计划项目(2009KS-12);延安大学科研计划项目(YD2008-04)。 |
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摘 要: | 背景:血管内皮生长因子及其受体在男性生殖领域中占有重要地位,但其在生殖系统中表达的意义和调节机制仍不十分清楚。
目的:观察血管内皮生长因子及其受体类fms酪氨酸激酶在青春期大鼠附睾内精子上的表达定位情况。
方法:取10只青春期雄性SD大鼠双侧附睾,分离得到浓度为(30~40)×109 L-1的精子,涂片,免疫荧光法检测精子上血管内皮生长因子及其受体类fms酪氨酸激酶的表达定位情况。
结果与结论:免疫荧光结果显示,血管内皮生长因子及其受体类fms酪氨酸激酶阳性蛋白均定位于大鼠附睾精子头部的顶体、尾部的颈段、中段和主段,尾部末段和精子核未见阳性染色。提示,血管内皮生长因子及其受体类fms酪氨酸激酶可能参与了精子的成熟过程,与精子的运动能力、获能和顶体反应有关。
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关 键 词: | 血管内皮生长因子 受体 类fms酪氨酸激酶 精子 免疫荧光 |
收稿时间: | 2011-02-14 |
Expression of vascular endothelial growth factor and its receptor in the epididymal sperm of adolescent male rats |
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Authors: | Ai Qing-yan Zhao Yu-feng Wang Yan-mei Miao Nai-zhou Ma Li Yang Jia-zhou |
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Institution: | Department of Human Histology and Embryology, Medical School of Yan’an University, Yan’an 716000, Shaanxi Province, China |
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Abstract: | BACKGROUND:Vascular endothelial growth factor (VEGF) and its receptor fms-like tyrosine kinase-1 (Flt-1) have a very important position in the field of male reproduction. However, it is still unclear about their expression meaning and regulatory mechanism in the reproductive system.
OBJECTIVE:To study the expression and location of VEGF and Flt-1 in the epididymal sperm of adolescent male rats.
METHODS:The expression of VEGF and Flt-1 was detected in 10 adolescent SD rats. The concentration of the sperm was (30-40)×109/L. Immunofluorescent staining was used for VEGF and Flt-1 expression and location in the sperm.
RESULTS AND CONCLUSION:Immunofluorescent staining showed that VEGF and Flt-1 were both localized in the acrosome of sperm head, as well as in the neck, middle and principal segment of sperm tail. Specific expression patterns of VEGF and Flt-1 in the sperm of rats display that they may participate in spermiotelcosis, relevant to movement, capacitation and acrosome reaction of the sperm. |
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