三七总皂苷干预股骨头缺血性坏死兔成骨细胞护骨素基因的表达

背景：前期研究已明确三七总皂苷能抑制乙醇诱导的兔骨髓基质干细胞成脂分化。 目的：进一步观察三七总皂苷对兔成骨细胞的增殖和分化以及骨保护素、细胞核因子κB受体活化因子配体mRNA表达的影响。 方法：白酒灌胃4周制备新西兰兔股骨头缺血坏死模型，随机分为生理盐水组、复方骨肽组和三七总皂苷组。通过组织块培养法提取各组兔成骨细胞，检测细胞的分化，骨保护素、细胞核因子κB受体活化因子配体基因表达情况，检测各目的基因杂交信号的强弱。 结果与结论：三七总皂苷组兔碱性磷酸酶活性、钙结节计数及骨保护素、细胞核因子κB受体活化因子配体的表达强度、骨保护素mRNA/细胞核因子κB受体活化因子配体mRNA均高于生理盐水组(P < 0.01)；与复方骨肽组效果接近。证实三七总皂苷能够促进兔成骨细胞的增殖和分化，并能提高成骨细胞的骨保护素mRNA的相对表达量而对其细胞核因子κB受体活化因子配体mRNA有抑制作用。

Total saponins of Panax notoginseng effects on mRNA expression of osteoprotegerin in osteoblasts from rabbits with avascular necrosis of the femoral head

BACKGROUND:Previous studies have demonstrated that total saponins of Panax notoginseng (PNS) can inhibit the ethanol-induced adipogenic differentiation of rabbit bone marrow stromal cells (BMSCs). OBJECTIVE:To observe PNS effects on osteoblast proliferation and mRNA expressions of osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL). METHODS: Rabbit models of alcohol-induced avascular necrosis of the femoral head were divided randomly into saline group, compound bone peptide group, PNS group. Rabbit osteoblasts were extracted for measurement of OPG and RANK mRNA expressions and hybridizing signal intensity. RESULTS AND CONCLUSION: In the PNS group, alkaline phosphatase activity, calcium nodule count, OPG and RANK mRNA expressions and their ratio were significantly higher than those in the saline group (P < 0.01), and similar to those in the compound bone peptide group. The experiment confirmed that PNS can promote the proliferation and differentiation of rabbit osteoblasts, and can improve the OPG mRNA relative expression in osteoblasts so as to inhibit RANKL mRNA expression.