激素性股骨头坏死模型兔血管内皮细胞生长因子表达与普伐他汀的干预

背景：有研究表明他汀类药物可使体外培养的人脐静脉内皮细胞的血管内皮细胞生长因子呈高表达，血管内皮细胞生长因子可促进内皮细胞增殖，明显提高成骨细胞活性并加速骨形成。目的：观察普伐他汀对激素性股骨头坏死兔模型血管内皮生长因子的蛋白表达的影响。方法：将新西兰白兔随机分为对照组，模型组和普伐他汀组。模型组和普伐他汀组制备兔激素性股骨头缺血坏死模型。普伐他汀组建模成功后以普伐他汀(1.2 mg/kg)灌胃，1次/d，模型组和对照组以等体积的蒸馏水灌胃。于造模后8，12，16周截取各组股骨头行免疫组织化学检测血管内皮生长因子蛋白的表达情况。结果与结论：对照组不同时间点股骨头血管内皮生长因子蛋白表达均为阳性。造模后8周，模型组血管内皮生长因子蛋白表达呈阴性表达，普伐他汀组呈弱阳性表达。造模后12周，模型组和普伐他汀组血管内皮生长因子蛋白表达呈阳性表达，16周呈弱阳性表达。结果证实，普伐他汀可有效促进早期激素性股骨头坏死兔模型坏死股骨头内源性血管内皮生长因子的蛋白表达。

Interventional effect of pravastatin on vascular endothelial growth factor expression in rabbits with steroid-induced avascular necrosis of the femoral head

BACKGROUND:Previous research has demonstrated that pravastatin can effectively promote endogenous vascular endothelial growth factor (VEGF) mRNA in endothelial cells from human umbilical vein cultured in vitro, but the research of protein VEGF expression in the femoral heads of rabbits with steroid-induced avascular necrosis of the femoral head (SANFH) after treatment with pravastatin is rare now.OBJECTIVE:To evaluate the endogenous protein VEGF expression in the femoral heads of SANFH rabbit models after treatment with pravastatin.METHODS:Totally 80 New Zealand white rabbits were randomly divided into a normal control group (n=18) and experimental group (n=62). Rabbits in the experimental group were prepared for SANFH models. At 5 weeks after model preparation, 36 rabbits in the model group were assigned into the model and pravastatin groups, with 18 animals in each group. Rabbits in the pravastatin group received intragastric administration with 1.2 mg/kg pravastatin once per day, and the same volume of distilled water was given to those in the model and control groups. Six rabbits in each group were killed at the 8th, 12th and 16th week respectively to evaluation of protein VEGF expression in the femoral heads by immunohistochemistry.RESULTS AND CONCLUSION: In the normal control group, endogenous expression of VEGF mRNA was positive at each time point. In the model group, 8 weeks after modeling, there was negative VEGF mRNA expression; 12 weeks after modeling positive expression; 16 weeks after modeling weakly positive expression. In the pravastatin group, 8 weeks after modeling, VEGF mRNA showed weak positive expression; 12 weeks after modeling positive expression; 16 weeks after modeling positive expression, but lower than 12 weeks after modeling slightly weakened. Pravastatin can effectively promote endogenous VEGF mRNA in early SANFH rabbit models.