前列腺素E1对人类脂肪源性基质细胞体外增殖的影响

背景：近年来脂肪源性基质细胞成为研究与应用的热点之一,但前列腺素E1对脂肪源性基质细胞增殖的作用影响尚未见报道。目的：观察前列腺素E1对人类脂肪源性基质细胞体外增殖的影响。方法：从人正常脂肪组织分离培养脂肪源性基质细胞,用5种不同浓度的前列腺素E1处理培养的细胞,其中3×10^-6,6×10^-6,9×10^-6,12×10^-6 mol/L作为实验组,0×10^-6 mol/L作为对照组,观察经不同浓度前列腺素E1处理后细胞的增殖情况。结果与结论：不同浓度实验组的细胞数量与对照组相比均显著升高,差异有显著性意义(P < 0.05),各实验组内相比,细胞数量增殖无显著性差异(P  > 0.05)。提示前列腺素E1具有促进脂肪源性基质细胞的增殖作用,且前列腺素E1的影响效果无剂量依赖性。

Effects of prostaglandin E1 on in vitro proliferation of human adipose derived stromal cells

BACKGROUND:Recently, research and application of human adipose derived stromal cells (ADSCs) has become a hotspot; however, there are few reports addressing prostaglandin E1 (PGE1) effects on ADSCs proliferation. OBJECTIVE:To explore the effects of PGE1 on ADSCs proliferation.METHODS:ADSCs were obtained from human normal adipose tissue after isolation and culture. The cultured ADSCs were treated with five different concentration of PGE1, which were 3×10^-6, 6×10^-6, 9×10^-6, 12×10^-6 mol/L, and 0×10^-6 mol/L (control group). After 3 days, the cell morphology and proliferation were observed.RESULTS AND CONCLUSION:There was no obervious different among the experimental groups. And the cell number of each experimental group was obervious elevated, compared with that of the control group (P < 0.05). These findings indicated that ① PGE1 promoted the proliferation of ADSC; ② The effect of PGE1 had no dose dependent.