耐力训练模型大鼠骨骼肌代谢酶与自由基变化

背景：骨骼肌代谢酶与自由基的变化与不同的运动方式和运动强度有关。 目的：观察递增大强度耐力训练下大鼠骨骼肌代谢酶活性和自由基代谢的变化。 方法：健康雄性SD大鼠,随机分为安静组和运动组,后者建立8周的递增大强度耐力训练模型,训练结束后取骨骼肌样本测试肌酸激酶、乳酸脱氢酶、谷草转氨酶、谷丙转氨酶、丙二醛、总抗氧化酶、过氧化氢酶水平。 结果与结论：8周训练后,运动组大鼠肌酸激酶、乳酸脱氢酶、谷草转氨酶、谷丙转氨酶酶活性及丙二醛水平均高于安静组(P < 0.01或0.05),而总抗氧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性低于安静组(P < 0.05)。说明8周的递增大强度的耐力训练能使骨骼肌受到一定的损伤,并且提示抗氧化酶活性的下降和部分代谢酶活性以升高之间有一定的关系。

Changes in skeletal muscle metabolic enzyme and free radical in a rat model of endurance training

BACKGROUND:Changes in skeletal muscle metabolic enzyme and free radical are related to different exercise modes and intensities. OBJECTIVE:To observe the changes in skeletal muscle metabolic enzyme and free radical in rats undergoing endurance exercise with gradually increased exercise intensities. METHODS:Healthy male Sprague-Dawley rats were randomly divided into a quiet control and an exercise group. Rats in the exercise group were subjected to 8 weeks of endurance exercise with gradually increased exercise intensities. After endurance exercise, skeletal muscle sample was taken to determine creatine kinase, lactic acid dehydrogenase, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase, total antioxidase, hydrogen peroxidase and glutathione peroxidase activities as well as malondialdehyde level. RESULTS AND CONCLUSION:In the exercise group, creatine kinase, lactic acid dehydrogenase, glutamic oxalacetic transaminase, and glutamic-pyruvic transaminase activity as well as malondialdehyde levels were significantly greater (P < 0.01 or P < 0.05), and total antioxidase, hydrogen peroxidase, and glutathione peroxidase activities were significantly lower (P < 0.05), compared with the quiet control group. Results showed that 8 weeks of endurance exercise with gradually increased exercise intensities can damage skeletal muscle to some extent, indicating that decreased activity of antioxidase is associated with increased activity of metabolic enzyme.