粒细胞集落刺激因子动员2型糖尿病患者外周血CD34+细胞的变化

背景：与正常人比较,2型糖尿病患者外周血CD34+细胞含量已有明显下降。目的：分析2型糖尿病患者外周血动员后CD34+细胞比例的变化。方法：将234例2型糖尿病患者按病程分为5组(<1年,1~5年,>5~<10年,10~15年和≥15年),予以粒细胞集落刺激因子动员。动员5 d后,使用流式细胞仪检测外周血CD34⁺细胞的含量,并利用Person简单相关及多元回归方法分析其与病程、血脂、尿酸的关系。结果与结论：糖尿病者外周血动员后CD34⁺细胞水平与三酰甘油相关(r=-0.202,P=0.002),与载脂蛋白B相关(r=-0.276,P=0.000),与尿酸相关(r=-0.297,P=0.000)。经统计分析发现,糖尿病者外周血动员后CD34⁺细胞数随糖尿病病程进展而逐渐下降。

Granulocyte colony-stimulating factor mobilizes peripheral blood CD34+ cells in patients with type 2 diabetes mellitus

BACKGROUND:The number of mobilized peripheral blood CD34⁺ cells significantly decreases in type 2 diabetes mellitus patients compared with normal subjects.OBJECTIVE:To investigate the changes of mobilized peripheral blood CD34⁺ cells in type 2 diabetes mellitus patients.METHODS:A total of 234 patients with type 2 diabetes mellitus were divided into five categories according to disease duration, newly diagnosed type 2 diabetes (≤1 year) and diabetes duration (1-5, 5-10, 10-15, ≥15 years). CD34⁺ cells were mobilized with granulocyte colony-stimulating factor. Five days later, the number of peripheral blood CD34⁺ cells was detected with flow cytometry. Its relationship with disease duration, blood lipids and uric acid was analyzed by person-related analysis and multiple regression analysis.RESULTS AND CONCLUSION: The number of mobilized peripheral blood CD34⁺ cells was associated with the triacylglycerol (r=-0.202, P=0.002), the apoprotein-B (r=-0.276, P=0.000), and the uric acid (r=-0.297, P=0.000). Statistical analysis found that, the number of mobilized peripheral blood CD34⁺ cells decreased with development of diabetes mellitus.