胶原-纳米羟基磷灰石复合支架的细胞相容性

背景：观察成骨细胞在生物材料上的形态、增殖和分化等项目,可评估生物支架材料的生物相容性。 目的：观察复合支架材料纳米羟基磷灰石/胶原对成骨细胞增殖、分化的影响。 方法：取新生24 h内Wistar大鼠的颅盖骨,采用改良胶原酶消化法进行成骨细胞原代培养,取第3代细胞与纳米羟基磷灰石/胶原支架或普通羟基磷灰石材料体外复合培养。培养3,6,9 d后,观察材料周边的细胞形态及支架材料对细胞分化、增殖的影响。 结果与结论：纳米羟基磷灰石/胶原材料较普通的羟基磷灰石材料更有利于成骨细胞的黏附、生长、分化、增殖,证实其生物相容性更好,有望成为一种新型的骨组织工程支架材料。

Cellular compatibility of a nano-hydroxyapatite/collagen scaffold

BACKGROUND:Under the theory of bone-tissue engineering, the biocompatibility of biomaterial scaffolds is evaluated by observing the morphology, proliferation and differentiation of osteoblasts on the biomaterials. OBJECTIVE:To observe the effects of nano-hydroxyapatite/collagen (nHAC) scaffold on the proliferation and differentiation of osteoblasts. METHODS:The rat osteoblasts were obtained from the cranium of newborn Wistar rats within 24 hours, and primarily cultured using modified collagenase digestion. The cells at passage 3 were co-cultured with nHAC in vitro. At 3, 6 and 9 days of the culture, cell morphology was observed by inverted phase-contrast microscopy. In addition, MTT assay and alkaline phosphatase (ALP) activity test were used to observe the effects of the material on cell differentiation and proliferation.  RESULTS AND CONCLUSION:Osteoblasts can adhere, grow, differentiate and proliferate better on nHAC than HA. The composite scaffold nHAC has a good compatibility, indicating that the material has a great potential for application in bone tissue engineering.