生长因子在脱钙骨基质上附着方法及存在的问题

背景：脱钙骨基质作为一种生物衍生骨组织工程支架材料,以其优良成骨性能受到越来越多的关注,然而脱钙骨固有的诱导蛋白较少,骨诱导活性有限。与生长因子复合后诱导骨组织生长的作用明显增强,有利于骨损伤及骨缺损患者的康复。 目的：综述生长因子在脱钙骨基质上的附着方法、研究进展及存在的主要问题。 方法：由第一作者检索PubMed数据库和万方数据库,英文关键词为“Demineralized bone”,“Growth factor”,“Combination”。中文关键词为“脱钙骨”,“生长因子”,“附着”。纳入脱钙骨、生长因子在医学上应用研究的相关文献以及生长因子与脱钙骨附着方法方面的相关文献。 结果与结论：脱钙骨具有良好的生物相容性、生物活性以及生物降解性,并且易于与周围骨融合,支持新骨组织的生长。将生长因子固定于脱钙骨上,能使生长因子准确、均匀到达骨缺损处,促进骨组织生长,更有利于骨移植和骨缺损患者的康复。目前脱钙骨基质与生长因子的复合方法仍存在结合不牢固,生长因子释放不稳定等问题,因此还需进一步加强对生长因子与脱钙骨基质复合方法的研究和探索。

Adhesion methods and problems of growth factors in demineralized bone matrix

BACKGROUND:As a bio-derived bone engineering scaffolds, demineralized bone matrix (DBM) become more and more popular for its bone formation performance. However, the inherent induced protein of demineralized bone are less, bone inducing activity is limited. The effect of induced bone issue growth was significantly enhanced after demineralized bone combined with growth factor, which is conductive to bone injury and the rehabilitation of patients with bone defects. OBJECTIVE:To summarize combination method, research progress and the main problems of growth factor in DBM. METHODS:PubMed (http://www.ncbi.nlm.nih.gov/PubMed) and Wanfang databases (http://www.wanfangdata.com.cn) were retrieved by the first author with the key words of “Demineralized bone, Growth factor, Combination” both in English and Chinese. Articles related to applied research of demineralized bone and growth factor in medicine and the relevant literature on growth factor and demineralized bone combination method were included. RESULTS AND CONCLUSION:The demineralized bone has excellent biocompatibility, biological activity and biodegradability, which can easily integrate with surrounding bone to support the growth of new bone tissue. The demineralized bone combined with the growth factor, not only can make the growth factor arrive to the bone defect accurately and uniformly, but also promote the growth of bone tissue and it will be more beneficial for bone transplantation and bone defects in rehabilitation of patients. At present, the composite method of the DBM combined with the growth factor still have some problems of loose combination and instable release of growth factors, etc. So the research and exploration of growth factor combined with DBM need to be further strengthened.