六味地黄丸含药血清对软骨终板蛋白多糖的影响

背景：软骨终板的结构完整性关系着其生理功能的正常发挥，进而影响整个椎间盘的生理、病理状态；蛋白多糖是软骨终板的主要组分。 目的：观察六味地黄丸含药血清对肿瘤坏死因子α致伤兔软骨终板蛋白多糖含量的影响。 方法：将12只新西兰白兔带终板的24个T12~L2椎间盘随机等分为对照第1，14天组、肿瘤坏死因子α组、肿瘤坏死因子α+中药血清组。后2组培养液中分别含5 μg/L肿瘤坏死因子α，5 μg/L肿瘤坏死因子α和体积分数10%六味地黄丸血清，培养14 d后观察。 结果与结论：随培养时间的延长，软骨终板细胞数量减少、细胞形态变化明显，细胞外基质中胶原水平减少，加入肿瘤坏死因子α，胶原成分下降更快，并出现大量裂隙，含药血清可部分延缓这一过程；随着培养时间延长，软骨终板超微结逐渐损伤，肿瘤坏死因子α可加重损伤，含药血清可部分抑制这种损伤；随着培养时间的延长，各组椎间盘软骨终板的糖胺多糖总量与硫酸软骨素、硫酸角质素和透明质酸水平均降低；肿瘤坏死因子α加速这种结果，而含药血清可延缓生化成分水平的下降。提示六味地黄丸可通过保护软骨终板超微结构，稳定糖胺多糖总量及其各成分水平，对椎间盘软骨终板具有保护作用。

Effects of liuwei dihuang wan-containing serum on proteoglycan content in cartilage endplates

BACKGROUND:Integrated cartilage endplate structure maintains its normal physiological function and then affects the physical and pathological conditions of the entire intervertebral disc. Proteoglycan is the main component of cartilage endplate. OBJECTIVE:To observe the effects of liuwei dihuang wan (LWDHW)-containing serum on proteoglycan content in cartilage endplates of tumor necorsis factor α (TNF-α)-induced injury rabbits. METHODS:Intervertebral discs with cartilage endplate were harvested from T12-L2 of 12 rabbits and then were randomly divided into four groups: control 1st day, control 14th day, TNF-α, and TNF-α + LWDHW-containing serum. In the TNF-α group, 5 μg/L TNF-α was added. In the TNF-α + LWDHW-containing serum group, 5 μg/L TNF-α and 10% LWDHW-containing serum were added. Then a 14-day culture was performed. RESULTS AND CONCLUSION:With prolongation of culture time, the number of cartilage endplates was decreased, cellular structure was obviously changed, and collagen content in extracellular matrix was decreased. With addition of TNF-α, collagen content decreased more faster and some crannies appeared. LWDHW-containing serum could delay this process. With prolongation of culture time, the ultrastructure of cartilage endplate was gradually destroyed, TNF-α could aggravate the damage, and LWDHW-containing serum could minimize the damage. With prolongation of culture time, proteoglycan content, chondroitin sulfate, keratan sulfate and hyaluronic acid levels in the cartilage endplates were declined in each group. The decline in the TNF-α group was most obvious (compared to the control group in 14th day, P < 0.05), and LWDHW-containing serum could postpone the decline of biochemical component levels (compared to TNF-α group, P < 0.05). These findings suggest that LWDHW-containing serum can protect the ultrastructure of cartilage endplate and stabilize the levels of biochemical components, thereby exhibiting protective effects on cartilage endplate of intervertebral discs.