静磁场作用下金属离子对成骨细胞的毒性

背景：体内体外实验研究证明,静磁场具有促进成骨细胞分化及骨形成的作用。目的：观察静磁场作用下金属离子对成骨细胞毒性的影响。方法：将CoCl₂粉末与CrCl₃粉末溶于无菌注射用水配制成CoCl₂溶液和CrCl₃溶液,将金属离子Co²⁺、Cr³⁺分别与小鼠颅骨成骨细胞(MC3T3-E1)在不同的磁场强度(1,10,100 mT)下共培养。实验分4组：设Co²⁺+Cr³⁺组(对照组)和Co²⁺+Cr³⁺组分别+1,10,100 mT组。结果与结论：恒磁场作用下的成骨细胞呈现更加成熟的形态学特征,Co²⁺、Cr³⁺金属离子对成骨细胞MC3T3的毒性明显降低;同时G2M(分裂期)分布比例明显增加,细胞停滞在G0G1(休眠期)的比率明显减少;与对照组相比,不同场强磁场加载后,成骨细胞碱性磷酸酶活性明显增强(P < 0.05)。提示一定强度的静磁场可拮抗Co²⁺、Cr³⁺金属离子对成骨细胞的毒性作用。

Static magnetic field reduces the toxic effect of metal ions on osteoblasts

BACKGROUND:Static magnetic field can promote the proliferation, differentiation and functional activity of osteoblasts.OBJECTIVE:To investigate the toxic effect of metal ions Co²⁺, Cr³⁺ on osteoblasts under the static magnetic field.METHODS:CoCl₂ powder and CrCl₃ powder were dissolved in the asepsis injecting water. Mouse calvarial osteoblasts (MC3T3-E1) were cultured with Co²⁺ and Cr³⁺ ions in different magnetic field of 1, 10, 100 mT. Cells only cultured with Co²⁺ and Cr³⁺ ions were taken as control group.RESULTS AND CONCLUSION:Under the static magnetic field, osteoblasts showed more mature morphological features; the toxic effect of metal ions Co²⁺ and Cr³⁺ on osteoblasts reduced significantly; G2M (dividing phase) distribution ratio increased and G0G1 (dormancy stage) distribution ratio decreased; the A value tested by alkaline phosphatase activity in the three experiment groups up-regulated obviously. The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co²⁺ and Cr³⁺ on osteoblasts.