| 不同保存方式下嗅鞘细胞的活性 |
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| 作者姓名： | 刘雨亮 魏开斌 刘 红 何玉芹 卓 峰 吕昕刚 |
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| 作者单位： | 泰安市中心医院,骨一科,护理部,山东省泰安市 271000;新汶矿务局莱芜中心医院骨一科,山东省莱芜市 271100 |
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| 基金项目： | 山东省医药卫生科技发展计划项目(课题编号:2009HW091)。 |
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| 摘 要： | 背景：获取和寻找适合的保存方式对嗅鞘细胞实验和临床应用有重要的意义。
目的：探索合适的嗅鞘细胞低温保存方式。
方法：取对数期生长的嗅鞘细胞,冻存1,3,6个月进行复苏。
结果与结论：MTT比色法及锥虫蓝染色显示5%二甲基亚砜-6%羟乙基淀粉处理的细胞活性最高,其次是10%二甲基亚砜处理的细胞,5%二甲基亚砜的保护作用最差。冰箱降温或程控降温仪降温方式及不同的冻存时间对嗅鞘细胞活性影响不明显。因此,推荐用5%二甲基亚砜-6%羟乙基淀粉作为嗅鞘细胞冻存低温保护剂。
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| 关 键 词： | 嗅鞘细胞 低温保护剂 二甲基亚砜 羟乙基淀粉 细胞活性 保存方式 细胞复苏 |
| 收稿时间： | 2011-01-19 |
Olfactory ensheathing cell viability in different cryopreservation systems |
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| Authors: | Liu Yu-liang Wei Kai-bin Liu Hong He Yu-qin Zhuo Feng Lü Xin-gang |
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| Institution: | First Department of Orthopedics, Nursing Department, Taian Central Hospital, Taian 271000, Shandong Province, China; First Department of Orthopedics, Xinwen Coal Mining Bureau Laiwu Central Hospital, Laiwu 271100, Shandong Province, China |
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| Abstract: | BACKGROUND:A proper preservation method would be of important significance for experiments and clinical application of olfactory ensheathing cells (OECs)
OBJECTIVE: To explore proper cyropreservative systems for OECs.
METHODS:OECs during the logarithmic growth phase were harvested, cryopreserved for 1, 3 and 6 months and then revitalized.
RESULTS AND CONCLUSION:MTT assay and tryplan blue staining showed that cells exhibited highest viability after treatment with 5% dimethyl sulfoxide (DMSO)-6% hydroxyethyl starch (HES), followed by 10% DMSO, and lastly the 5% DMSO. Use of refrigerator or cryogenic control system with different cryopreservation time did not yield obvious effects on viability of OECs. Therefore, 5% DMSO-6%HES is recommended as a cryopreservative agent for OECs. |
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