基质金属蛋白酶13和转化生长因子β1在瘢痕疙瘩和增生性瘢痕中的表达

背景：与瘢痕疙瘩和增生性瘢痕发生机制相关的基质金属蛋白酶13和转化生长因子β1信号传递通路研究多集中在体外成纤维细胞的培养上,而在组织中的相关研究少见报道。 目的：观察瘢痕疙瘩和增生性瘢痕中基质金属蛋白酶13和转化生长因子β1蛋白的表达。 方法：取自2004/2008唐山市工人医院烧伤整形科手术患者,瘢痕疙瘩54例,增生性瘢痕42例。选取同期45例因非感染手术切除的正常瘢痕组织作为对照组,选取同期45例正常皮肤组织作为正常对照组。应用流式细胞仪检测4组中基质金属蛋白酶13和转化生长因子β1的表达,分析两者的相关性。 结果与结论：瘢痕疙瘩和增生性瘢痕中转化生长因子β1的表达明显高于正常瘢痕组织和正常皮肤组织;正常瘢痕组织中转化生长因子β1的表达明显则高于正常皮肤组织,而基质金属蛋白酶13的表达与之相反。瘢痕疙瘩、增生性瘢痕和正常瘢痕组织中基质金属蛋白酶13和转化生长因子β1表达呈负相关。由此推测基质金属蛋白酶13和转化生长因子β1在瘢痕组织中异常表达,二者可能具有协同负向作用,共同参与病理性瘢痕的发生发展。

Expressions of matrix metalloproteinase 13 and transforming growth factor beta 1 in keloid and hypertrophic scars

BACKGROUND:Studies regarding matrix metalloproteinase 13 (MMP-13) and transforming growth factor-β1 (TGF-β1) signaling pathways in pathological scars are mainly focused on in vitro culture of fibroblasts; however, the correlation studies are rare on tissue of operation. OBJECTIVE:To observe the expressions of MMP-13 and TGF-β1 in keloid and hypertrophic scars. METHODS:Experimental samples were obtained from the patients, who underwent burn and plastic surgery at the Department of Burn and Plastic Surgery, Workers’ Hospital of Tangshan, from June 2004 to June 2008, including 54 patients with keloid, and 42 patients with hypertrophic scars. Normal scar from additional 45 cases were served as control group, normal skins from additional 40 cases were served as normal controls. The expressions of MMP-13 and TGF-β1 protein in keloid, hypertrophic scars, normal scar and normal skin were examined by flow cytometry. RESULTS:The expression of TGF-β1 was obviously greater in the experimental group (keloid and hypertrophic scars) than the control group (normal scar and normal skin) (P < 0.05). The expression of MMP-13 was obviously lower in the experimental group (keloid and hypertrophic scars) than the control group (normal scar and normal skin) (P < 0.05), but the difference between keloid and hypertrophic scars had no significance (P > 0.05). There was a negative correlation between MMP-13 and TGF-β1 in keloid, hypertrophic scars and normal scars (r=0.47, r=0.43, r=0.45; P < 0.05). No notable correlation was found between MMP-13 and TGF-β1 in normal skins (P > 0.05). It is indicated that the expressions of MMP-13 and TGF-β1 are changed, and the synergism of MMP-13 and TGF-β1 may promote the development in pathological scars.