血小板衍化生长因子β对体外人牙周膜细胞增殖分化的影响

背景：牙周支持组织破坏及附着丧失是导致失牙的最主要原因，细胞因子能促进牙周组织再生形成新附着。目的：观察血小板衍化生长因子β在人牙周膜成纤维细胞增殖过程中的作用。方法：体外利用组织块法培养人牙周膜成纤维细胞，对传代后细胞进行组织来源鉴定，通过波丝蛋白、角蛋白免疫组化染色进行定性分析，并加入生物因子血小板衍化生长因子β对人牙周膜成纤维细胞进行增殖诱导，采用MTT法测定吸光度值。结果与结论：加入质量浓度为10 μg/L的血小板衍化生长因子β后，细胞增殖加速，在加入生长因子血小板衍化生长因子β后，实验组的吸光度显著高于对照组，说明血小板衍化生长因子β对人牙周膜成纤维细胞的增殖具有明显的促进作用。

Effect of platelet-derived growth factor beta on in vitro proliferation and differentiation of human periodontal ligament cells

BACKGROUND:Periodontal supporting tissues destroy and attachment losses are the main reasons for tooth loss. Cytokine can promote periodontal tissue regeneration. OBJECTIVE:To research the effect of platelet-derived growth factor-β (PDGF-β) in the human periodontal ligament cells proliferation.METHODS:Human fibroblasts were cultured with tissue block method in vitro. The passaged cells were identified by Bosi protein, keratin immunohistochemistry for qualitative analysis. PDGF-β was added to induce periodontal ligament cells proliferation, and the absorbance value was measured by MTT method.RESULTS AND CONCLUSION:The cell proliferation was accelerated after adding 10 μg/L PDGF-β factor, the absorbance value of the experimental group was greater than that of the control group (P < 0.05). The findings demonstrated that PDGF-β can promote the proliferation of human periodontal ligament cells.